SLC30A8 Antibody; Solute carrier family 30 member 8; zinc transporter 8; ZNT8; Zn-T8

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

Two isoforms of SLC30A8 are known to exist; this antibody will detect both isoforms. This SLC30A8 antibody is predicted to not cross-react with other members of the SLC30 protein family.

Affinity chromatography purified via peptide column.

Liquid

1 mg/mL (lot specific)

Store at 4 degree C for three months and -20 degree C, stable for up to one year.
As with all antibodies care should be taken to avoid repeated freeze thaw cycles
Antibodies should not be exposed to prolonged high temperatures.

Small volumes of anti-SLC30A8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

SLC30A8 Antibody: SLC30A8 is a zinc efflux transporter involved in the accumulation of zinc in intracellular vesicles and is expressed at a high level in the pancreas, particularly in islets of Langerhans, and co-localizes with insulin in the secretory pathway granules of the insulin-secreting INS-1 cells (1). Allelic variants of SLC30A8 have been identified as major genetic risk factors for the development of Type 2 diabetes (2), but no genetic association has been found with Type 1 diabetes (3). SLC30A8 expression in ?-cells was found to be influenced by cytokine expression, particularly IFN-gamma and IL-1beta (4).

Homeostasis

ELISA (EIA), Western Blot (WB)

Western blot at 1 - 2 mug/mL.

Western blot analysis of SLC30A8 in human pancreas tissue lysate with SLC30A8 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide

Immunohistochemistry of SLC30A8 in human pancreas tissue with SLC30A8 Antibody at 5 μg/mL.

Immunofluorescence of SLC30A8 in human pancreas tissue with SLC30A8 Antibody at 20 μg/mL.

Predicted: 35, 41 kDa
Observed: 35, 41 kDa

solute carrier family 30 member 8

zinc transporter 8

Zinc transporter 8

ZNT8; ZnT-8??[Similar Products]

The protein encoded by this gene is a zinc efflux transporter involved in the accumulation of zinc in intracellular vesicles. This gene is expressed at a high level only in the pancreas, particularly in islets of Langerhans. The encoded protein colocalizes with insulin in the secretory pathway granules of the insulin-secreting INS-1 cells. Allelic variants of this gene exist that confer susceptibility to diabetes mellitus, noninsulin-dependent (NIDDM). Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2010]

SLC30A8: Facilitates the accumulation of zinc from the cytoplasm into intracellular vesicles, being a zinc-efflux transporter. May be a major component for providing zinc to insulin maturation and/or storage processes in insulin-secreting pancreatic beta- cells. Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. SLC30A subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Transporter; Transporter, SLC family; Vesicle

Chromosomal Location of Human Ortholog: 8q24.11

Cellular Component: cytoplasmic vesicle; Golgi apparatus; plasma membrane; secretory granule; secretory granule membrane

Molecular Function: protein binding; protein homodimerization activity; zinc ion binding; zinc ion transmembrane transporter activity

Biological Process: cellular zinc ion homeostasis; glucose homeostasis; insulin secretion; positive regulation of insulin secretion; response to glucose stimulus; response to zinc ion; sequestering of zinc ion; zinc ion transport

Disease: Diabetes Mellitus, Noninsulin-dependent

1. Chimienti F, Devergnas S, Favier A, et al. Identification and cloning of a beta-cell-specific zinc transporter, ZnT-8, localized into insulin secretory granules. Diabetes 2004; 53:2330-7. 2. Sladek R, Rocheleau G, Rung J, et al. A genome-wide association study indentifies novel risk loci for type 2 diabetes. Science 2007; 445:881-5. 3. Qu HQ, Grant SF, Bradfield JP, et al. Association analysis of type 2 diabetes Loci in type 1 diabetes. Diabetes 2008; 57:1983-6. 4. Egefjord L, Jensen JL, Bang-Berthelsen CH, et al. Zinc transporter gene expression is regulated by pro-inflammatory cytokines: a potential role for zinc transporters in beta-cell apoptosis? BMC Endocrine Disorders 2009; 9:7.

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