Product Name
Keratin 3 (KRT3), Recombinant Protein
Full Product Name
Recombinant Keratin 3 (KRT3)
Product Gene Name
KRT3 recombinant protein
[Similar Products]
Matching Pairs
Unconjugated
Antibody: Keratin 3 (MBS2028366)
Immunogen: Keratin 3 (MBS2031496)
Matching Pairs
APC-CY7 Conjugated Antibody: Keratin 3 (KRT3) (MBS2043031)
Immunogen: Keratin 3 (MBS2031496)
Matching Pairs
PE Conjugated Antibody: Keratin 3 (KRT3) (MBS2043032)
Immunogen: Keratin 3 (MBS2031496)
Matching Pairs
APC Conjugated Antibody: Keratin 3 (KRT3) (MBS2043033)
Immunogen: Keratin 3 (MBS2031496)
Matching Pairs
Cy3 Conjugated Antibody: Keratin 3 (KRT3) (MBS2043034)
Immunogen: Keratin 3 (MBS2031496)
Matching Pairs
FITC Conjugated Antibody: Keratin 3 (KRT3) (MBS2043035)
Immunogen: Keratin 3 (MBS2031496)
Matching Pairs
HRP Conjugated Antibody: Keratin 3 (KRT3) (MBS2043036)
Immunogen: Keratin 3 (MBS2031496)
Matching Pairs
Biotin Conjugated Antibody: Keratin 3 (KRT3) (MBS2092064)
Immunogen: Keratin 3 (MBS2031496)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHH H SSGLVPRGSH MASMTGGQQM GRGSEF- ERE QIKTLNNKFA SFIDKVRFLE QQNKVLETKW NLLQQQGTSS ISGTNNLEPL FENHINYLRS YLDNILGERG RLDSELKNME DLVEDFKKKY EDEINKRTAA ENEFVTLKKD VDSAYMNKVE LQAKVDALID EIDFLRTLYD AELSQMQSHI SDTSVVLSMD NNRSLDLDSI IAEVRAQYED IAQRSKAEAE ALYQTKLGEL QTTAGRHGDD LRNTKSEIIE LNRMIQRLRA EIEGVKKQNA NLQTAIAEAE QHGEMALKDA NAKLQELQAA LQQAKDDLAR LLRDYQELMN VKLALDVEIA TYRKLLEGEE YRMS
3D Structure
ModBase 3D Structure for P12035
Host
Host: E Coli
Source: Prokaryotic expression
Form/Format
Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Concentration
5.3 (lot specific)
Predicted Molecular Mass
40.3kDa
Accurate Molecular Mass (KD)
40kDa
Endotoxin
<1.0EU per 1ug (determined by the LAL method)
Expression System
Prokaryotic expression
Tag
two N-terminal Tags, His-tag and T7-tag
Organism Species
Homo sapiens (Human)
Usage
Reconstitute in sterile PBS, pH7.2-pH7.4.
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customera??s specifications, please inquire.
Other Notes
Small volumes of KRT3 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for KRT3 recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
SDS-Page of KRT3 recombinant protein
NCBI/Uniprot data below describe general gene information for KRT3. It may not necessarily be applicable to this product.
NCBI Accession #
CAF31522.1
[Other Products]
UniProt Primary Accession #
P12035
[Other Products]
UniProt Secondary Accession #
Q701L8; A6NIS2[Other Products]
Molecular Weight
64,417 Da
NCBI Official Full Name
keratin 3
UniProt Protein Name
Keratin, type II cytoskeletal 3
UniProt Synonym Protein Names
65 kDa cytokeratin; Cytokeratin-3; CK-3; Keratin-3; K3; Type-II keratin Kb3
UniProt Gene Name
KRT3??[Similar Products]
UniProt Synonym Gene Names
CK-3; K3??[Similar Products]
UniProt Entry Name
K2C3_HUMAN
UniProt Comments for KRT3
K3: a type II cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly. Generally associates with K12.
Protein type: Cytoskeletal
Chromosomal Location of Human Ortholog: 12q13.13
Cellular Component: keratin filament; intermediate filament
Molecular Function: structural molecule activity
Biological Process: epithelial cell differentiation; intermediate filament cytoskeleton organization and biogenesis
Disease: Corneal Dystrophy, Meesmann
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
