Product Name
ACY1/Aminoacylase-1, Monoclonal Antibody
Full Product Name
Mouse anti Human ACY1/Aminoacylase-1 Monoclonal Antibody
Product Gene Name
anti-ACY1 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen
Recombinant Human ACY1 protein
Buffer
0.2 mum filtered solution in PBS with 5% trehalose
Preparation and Storage
This antibody can be stored at 2 degree C- 8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-ACY1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-ACY1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-ACY1 antibody
WB: 5-10 mug/mL
ELISA: 0.5-1 mug/mL. This antibody can be used at 0.5-1 mug/mL with the appropriate secondary reagents to detect Human ACY1. The detection limit for Human ACY1 is 0.039 ng/well.
NCBI/Uniprot data below describe general gene information for ACY1. It may not necessarily be applicable to this product.
UniProt Secondary Accession #
C9J6I6; C9J9D8; C9JWD4[Other Products]
UniProt Related Accession #
Q03154[Other Products]
Molecular Weight
42,195 Da
NCBI Official Full Name
aminoacylase-1
NCBI Official Synonym Full Names
aminoacylase 1
NCBI Official Symbol
ACY1??[Similar Products]
NCBI Official Synonym Symbols
ACY-1; ACY1D; HEL-S-5
??[Similar Products]
NCBI Protein Information
aminoacylase-1
UniProt Protein Name
Aminoacylase-1
UniProt Synonym Protein Names
N-acyl-L-amino-acid amidohydrolase
Protein Family
Aminoacylase
UniProt Gene Name
ACY1??[Similar Products]
UniProt Synonym Gene Names
ACY-1??[Similar Products]
UniProt Entry Name
ACY1_HUMAN
NCBI Summary for ACY1
This gene encodes a cytosolic, homodimeric, zinc-binding enzyme that catalyzes the hydrolysis of acylated L-amino acids to L-amino acids and an acyl group, and has been postulated to function in the catabolism and salvage of acylated amino acids. This gene is located on chromosome 3p21.1, a region reduced to homozygosity in small-cell lung cancer (SCLC), and its expression has been reported to be reduced or undetectable in SCLC cell lines and tumors. The amino acid sequence of human aminoacylase-1 is highly homologous to the porcine counterpart, and this enzyme is the first member of a new family of zinc-binding enzymes. Mutations in this gene cause aminoacylase-1 deficiency, a metabolic disorder characterized by central nervous system defects and increased urinary excretion of N-acetylated amino acids. Alternative splicing of this gene results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream ABHD14A (abhydrolase domain containing 14A) gene, as represented in GeneID:100526760. A related pseudogene has been identified on chromosome 18. [provided by RefSeq, Nov 2010]
UniProt Comments for ACY1
ACY1: Involved in the hydrolysis of N-acylated or N-acetylated amino acids (except L-aspartate). Defects in ACY1 are the cause of aminoacylase-1 deficiency (ACY1D). ACY1D results in a metabolic disorder manifesting with encephalopathy, unspecific psychomotor delay, psychomotor delay with atrophy of the vermis and syringomyelia, marked muscular hypotonia or normal clinical features. Epileptic seizures are a frequent feature. All affected individuals exhibit markedly increased urinary excretion of several N-acetylated amino acids. Belongs to the peptidase M20A family.
Protein type: Amino Acid Metabolism - arginine and proline; EC 3.5.1.14; Hydrolase
Chromosomal Location of Human Ortholog: 3p21.1
Cellular Component: cytosol
Molecular Function: aminoacylase activity; identical protein binding; protein binding; zinc ion binding
Biological Process: peptide catabolic process; xenobiotic metabolic process
Disease: Aminoacylase 1 Deficiency
Research Articles on ACY1
1. Biochemical analysis showed absence of ACY1 enzyme activity in the patient's fibroblasts.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
