Product Name
Sclerostin (SOST), Recombinant Protein
Full Product Name
Recombinant Human Sclerostin, HEK
Product Synonym Names
Sclerostin; SOST; CDD; VBCH; SOST Human, HEK; Sclerostin Human Recombinant, HEK
Product Gene Name
SOST recombinant protein
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence
QGWQAFKNDA TEIIPELGEY PEPPPELENN KTMNRAENGG RPPHHPFETK DVSEYSCREL HFTRYVTDGP CRSAKPVTEL VCSGQCGPAR LLPNAIGRGK WWRPSGPDFR CIPDRYRAQR VQLLCPGGEA PRARKVRLVA SCKCKRLTRF HNQSELKDFG TEAARPQKGR KPRPRARSAK ANQAELENAY HHHHHH
3D Structure
ModBase 3D Structure for Q9BQB4
Purity/Purification
Greater than 95.0% as determined by SDS-PAGE
Form/Format
Filtered white lyophilized (freeze-dried) powder
Filtered (0.4 um) and lyophilized from 0.5mg/ml in PBS and 5 % (w/v) trehalose, pH 7.4.
Solubility
It is recommended to add 200ul deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. SOST is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture
Preparation and Storage
Store lyophilized protein at-20 degree C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles.
Reconstituted protein can be stored at 4 degree C for a limited period of time; it does not show any change after one week at 4 degree C
Other Notes
Small volumes of SOST recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
SOST recombinant protein
Description: SOST Human Recombinant produced in HEK293 cells is a single, glycosylated polypeptide chain (a.a 24-213) containing 196 amino acids including a 6 a.a C-terminal His tag. The total molecular mass is 22.4kDa (calculated).
Introduction: Sclerostin (SOST) is a secreted glycoprotein with a C-terminal cysteine knot-like (CTCK) domain and sequence similarity to the DAN (differential screening-selected gene aberrative in neuroblastoma) family of bone morphogenetic protein (BMP) antagonists. Sclerostin functions as a negative regulator of bone growth, by inhibiting bone formation. SOST is widely expressed at low levels, with highest levels in the bone, cartilage, kidney, liver, bone marrow and primary osteoblasts differentiated for 21 days. SOST gene defects cause sclerosteosis and bone dysplasia.
Product Categories/Family for SOST recombinant protein
RECOMBINANT & NATURAL PROTEINS; Recombinant Proteins; Sclerostin; SOST Human, HEK
NCBI/Uniprot data below describe general gene information for SOST. It may not necessarily be applicable to this product.
NCBI Accession #
NP_079513.1
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NCBI GenBank Nucleotide #
NM_025237.2
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UniProt Primary Accession #
Q9BQB4
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UniProt Secondary Accession #
Q495N9[Other Products]
UniProt Related Accession #
Q9BQB4[Other Products]
Molecular Weight
24,264 Da
NCBI Official Full Name
sclerostin
NCBI Official Synonym Full Names
sclerostin
NCBI Official Symbol
SOST??[Similar Products]
NCBI Official Synonym Symbols
CDD; VBCH; DAND6; SOST1
??[Similar Products]
NCBI Protein Information
sclerostin
UniProt Protein Name
Sclerostin
Protein Family
Sclerostin
UniProt Gene Name
SOST??[Similar Products]
NCBI Summary for SOST
Sclerostin is a secreted glycoprotein with a C-terminal cysteine knot-like (CTCK) domain and sequence similarity to the DAN (differential screening-selected gene aberrative in neuroblastoma) family of bone morphogenetic protein (BMP) antagonists. Loss-of-function mutations in this gene are associated with an autosomal-recessive disorder, sclerosteosis, which causes progressive bone overgrowth. A deletion downstream of this gene, which causes reduced sclerostin expression, is associated with a milder form of the disorder called van Buchem disease. [provided by RefSeq, Jul 2008]
UniProt Comments for SOST
Negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation.
Research Articles on SOST
1. Positivity of RANKL and anti-CCP2 yielded significant risk for progression with negativity for both as reference. No single nucleotide polymorphism encoding TNFSF11 or SOST was associated with increased concentrations of the factors.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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