Mouse Acyloxyacyl hydrolase (AOAH) ELISA kit; acyloxyacyl hydrolase; acyloxyacyl hydrolase (neutrophil)

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of Mouse AOAH. No significant cross-reactivity or interference between Mouse AOAH and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of AOAH elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS903961 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the acyloxyacyl hydrolase (neutrophil) (AOAH) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing AOAH. The ELISA analytical biochemical technique of the MBS903961 kit is based on AOAH antibody-AOAH antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AOAH antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AOAH. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for AOAH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AOAH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AOAH is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AOAH bound in the initial step. The color development is stopped and the intensity of the color is measured.

65,155 Da

acyloxyacyl hydrolase

acyloxyacyl hydrolase

Acyloxyacyl hydrolase

AOAH_MOUSE

This genes encodes an enzyme that catalyzes the hydrolysis of acyloxylacyl-linked fatty acyl chains from bacterial lipopolysaccharides. The encoded protein modulates host inflammatory response to gram-negative bacteria. The proprotein is further cleaved into a large and small chain that interact in a heterodimer. Alternative splicing results in multiple transcript variants for this gene. [provided by RefSeq, Aug 2013]

AOAH: Removes the secondary (acyloxyacyl-linked) fatty acyl chains from the lipid A region of bacterial lipopolysaccharides. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.1.77; Secreted, signal peptide; Secreted; Lipase

Cellular Component: extracellular region

Molecular Function: acyloxyacyl hydrolase activity; hydrolase activity; hydrolase activity, acting on ester bonds

Biological Process: lipopolysaccharide metabolic process; negative regulation of inflammatory response

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