Goat Anti-cardiac troponin T Antibody; TNNT2; troponin T type 2 (cardiac); CMH2; CMPD2; LVNC6; MGC3889; RCM3; TnTC; cTnT; OTTHUMP00000033864; OTTHUMP00000033865; OTTHUMP00000033866; OTTHUMP00000033867; OTTHUMP00000033870; OTTHUMP00000218095; cardiac muscle troponin T; cardiomyopathy, dilated 1; cardiac muscle troponin T antibody; cardiomyopathy; dilated 1D (autosomal dominant) antibody; cardiomyopathy; hypertrophic 2 antibody; CMH2 antibody; CMPD2 antibody; cTnT antibody; LVNC6 antibody; MGC3889 antibody; OTTHUMP00000033864 antibody; OTTHUMP00000033865 antibody; OTTHUMP00000033866 antibody; OTTHUMP00000033867 antibody; OTTHUMP00000033870 antibody; OTTHUMP00000218095 antibody; RCM3 antibody; TNNT2 antibody; TnTC antibody; troponin T type 2 (cardiac) antibody; troponin T; cardiac muscle antibody; troponin T2; cardiac antibody; Cardiac troponin T; cardiac troponin T (aa201-213)

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Goat

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul (lot specific)

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Manufactured in an ISO 9001:2008 Certified Laboratory.

Small volumes of anti-TNNT2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Peptide ELISA (EIA), Western Blot (WB)

Peptide ELISA: Antibody detection limit dilution 1: 128000.
Western Blot: Approx 40kDa band observed in Human, Mouse and Pig Heart lysates (calculated MW of 35.6kDa according to Human NP_000355.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Luedde, Cardiovasc Res.2010 Jun 1;86(3): 452-60. PMID: 20083571). Recommended concentration: 0.1-0.3ug/ml.

(0.1ug/ml) staining of Human (A) and Mouse (B) Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

(0.1ug/ml) staining of Pig Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

30,681 Da

troponin T2, cardiac type

troponin T, cardiac muscle

Troponin T, cardiac muscle

TnTc; cTnT??[Similar Products]

TNNT2_HUMAN

The protein encoded by this gene is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for this gene undergo alternative splicing that results in many tissue-specific isoforms, however, the full-length nature of some of these variants has not yet been determined. [provided by RefSeq, Jul 2008]

TNNT2: Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the ***** heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal ***** heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing ***** heart. Belongs to the troponin T family. 11 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Motor

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: cytosol; sarcomere; striated muscle thin filament; troponin complex

Molecular Function: actin binding; ATPase activity; protein binding; protein binding, bridging; tropomyosin binding; troponin C binding; troponin I binding

Biological Process: actin crosslink formation; cardiac muscle contraction; muscle filament sliding; negative regulation of ATPase activity; positive regulation of ATPase activity; protein heterooligomerization; regulation of heart contraction; regulation of muscle filament sliding speed; response to calcium ion; ventricular cardiac muscle morphogenesis

Disease: Cardiomyopathy, Dilated, 1d; Cardiomyopathy, Familial Hypertrophic, 2; Cardiomyopathy, Familial Restrictive, 3

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