Full Product Name
CNST siRNA (Human)
Product Synonym Names
C1orf71; Consortin
Product Gene Name
CNST sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q6PJW8
Specificity
CNST siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CNST gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of CNST sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
CNST sirna
siRNA to inhibit CNST expression using RNA interference
Applications Tested/Suitable for CNST sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for CNST. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001132931.1
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NCBI GenBank Nucleotide #
NM_001139459.1
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UniProt Primary Accession #
Q6PJW8
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UniProt Secondary Accession #
Q5VSY9; Q5VTM7; Q8IYA9; Q8N3L5; Q8NB09; Q8TEI2; Q96MR5[Other Products]
UniProt Related Accession #
Q6PJW8[Other Products]
Molecular Weight
29,306 Da
NCBI Official Full Name
consortin isoform 2
NCBI Official Synonym Full Names
consortin, connexin sorting protein
NCBI Official Symbol
CNST??[Similar Products]
NCBI Official Synonym Symbols
C1orf71; PPP1R64
??[Similar Products]
NCBI Protein Information
consortin
UniProt Protein Name
Consortin
UniProt Gene Name
CNST??[Similar Products]
UniProt Synonym Gene Names
C1orf71??[Similar Products]
UniProt Entry Name
CNST_HUMAN
NCBI Summary for CNST
Targeting of numerous transmembrane proteins to the cell surface is thought to depend on their recognition by cargo receptors that interact with the adaptor machinery for anterograde traffic at the distal end of the Golgi complex. Consortin (CNST) is an integral membrane protein that acts as a binding partner of connexins, the building blocks of gap junctions, and acts as a trans-Golgi network (TGN) receptor involved in connexin targeting to the plasma membrane and recycling from the cell surface (del Castillo et al., 2010 [PubMed 19864490]).[supplied by OMIM, Jun 2010]
UniProt Comments for CNST
CNST: Required for targeting of connexins to the plasma membrane. Belongs to the CNST family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 1q44
Cellular Component: transport vesicle; protein complex; membrane; integral to membrane; plasma membrane; trans-Golgi network
Molecular Function: protein binding; phosphatase binding
Biological Process: positive regulation of Golgi to plasma membrane protein transport
Research Articles on CNST
1. Consortin is a trans-Golgi network cargo receptor for the plasma membrane targeting and recycling of connexins.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
