Product Name
Dynamin-like 120 kDa protein (OPA1), Recombinant Protein
Full Product Name
Recombinant Human Dynamin-like 120 kDa protein, mitochondrial (OPA1)
Product Gene Name
OPA1 recombinant protein
[Similar Products]
Product Synonym Gene Name
KIAA0567[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Positions
195-960aa; Full length protein
Sequence
ATDRGSESDK HFRKVSDKEK IDQLQEELLH TQLKYQRILE RLEKENKELR KLVLQKDDKG IHHRKLKKSL IDMYSEVLDV LSDYDASYNT QDHLPRVVVV GDQSAGKTSV LEMIAQARIF PRGSGEMMTR SPVKVTLSEG PHHVALFKDS SREFDLTKEE DLAALRHEIE LRMRKNVKEG CTVSPETISL NVKGPGLQRM VLVDLPGVIN TVTSGMAPDT KETIFSISKA YMQNPNAIIL CIQDGSVDAE RSIVTDLVSQ MDPHGRRTIF VLTKVDLAEK NVASPSRIQQ IIEGKLFPMK ALGYFAVVTG KGNSSESIEA IREYEEEFFQ NSKLLKTSML KAHQVTTRNL SLAVSDCFWK MVRESVEQQA DSFKATRFNL ETEWKNNYPR LRELDRNELF EKAKNEILDE VISLSQVTPK HWEEILQQSL WERVSTHVIE NIYLPAAQTM NSGTFNTTVD IKLKQWTDKQ LPNKAVEVAW ETLQEEFSRF MTEPKGKEHD DIFDKLKEAV KEESIKRHKW NDFAEDSLRV IQHNALEDRS ISDKQQWDAA IYFMEEALQA RLKDTENAIE NMVGPDWKKR WLYWKNRTQE QCVHNETKNE LEKMLKCNEE HPAYLASDEI TTVRKNLESR GVEVDPSLIK DTWHQVYRRH FLKTALNHCN LCRRGFYYYQ RHFVDSELEC NDVVLFWRIQ RMLAITANTL RQQLTNTEVR RLEKNVKEVL EDFAEDGEKK IKLLTGKRVQ LAEDLKKVRE IQEKLDAFIE ALHQEK
3D Structure
ModBase 3D Structure for O60313
Host
Cell Free Expression
Form/Format
Liquid containing glycerol
Storage Buffer
Tris-based buffer, 50% glycerol.
Preparation and Storage
Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.
Repeated freezing and thawing is not recommended. Store working aliquots at 4 degree C for up to one week.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of OPA1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for OPA1 recombinant protein
Transmembrane Protein
Application Notes for OPA1 recombinant protein
This is a recombinant transmembrane protein expressed in a cell-free expression system.
NCBI/Uniprot data below describe general gene information for OPA1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_056375.2
[Other Products]
NCBI GenBank Nucleotide #
NM_015560.2
[Other Products]
UniProt Primary Accession #
O60313
[Other Products]
UniProt Secondary Accession #
D3DNW4[Other Products]
UniProt Related Accession #
O60313[Other Products]
Molecular Weight
115,884 Da
NCBI Official Full Name
dynamin-like 120 kDa protein, mitochondrial isoform 1
NCBI Official Synonym Full Names
OPA1, mitochondrial dynamin like GTPase
NCBI Official Symbol
OPA1??[Similar Products]
NCBI Official Synonym Symbols
NPG; NTG; MGM1; BERHS; largeG; MTDPS14
??[Similar Products]
NCBI Protein Information
dynamin-like 120 kDa protein, mitochondrial
UniProt Protein Name
Dynamin-like 120 kDa protein, mitochondrial
UniProt Synonym Protein Names
Optic atrophy protein 1
Protein Family
Dynamin-like 120 kDa protein
UniProt Gene Name
OPA1??[Similar Products]
UniProt Synonym Gene Names
KIAA0567??[Similar Products]
UniProt Entry Name
OPA1_HUMAN
NCBI Summary for OPA1
This gene product is a nuclear-encoded mitochondrial protein with similarity to dynamin-related GTPases. It is a component of the mitochondrial network. Mutations in this gene have been associated with optic atrophy type 1, which is a dominantly inherited optic neuropathy resulting in progressive loss of visual acuity, leading in many cases to legal blindness. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
UniProt Comments for OPA1
OPA1: a dynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space. Defects in OPA1 are the cause of optic atrophy 1 and deafness. Eight splice-variant isoforms have been described.
Protein type: Mitochondrial; Hydrolase; EC 3.6.5.5; Membrane protein, integral; Apoptosis
Chromosomal Location of Human Ortholog: 3q29
Cellular Component: cytoplasm; dendrite; integral to membrane; membrane; mitochondrial crista; mitochondrial inner membrane; mitochondrial intermembrane space; mitochondrial outer membrane; mitochondrion; nucleoplasm
Molecular Function: GTP binding; GTPase activity; magnesium ion binding; protein binding
Biological Process: apoptosis; axon transport of mitochondrion; inner mitochondrial membrane organization and biogenesis; metabolic process; mitochondrial fission; mitochondrial fusion; mitochondrial genome maintenance; mitochondrion organization and biogenesis; positive regulation of neuron maturation; visual perception
Disease: Glaucoma, Normal Tension, Susceptibility To; Optic Atrophy 1; Optic Atrophy With Or Without Deafness, Ophthalmoplegia, Myopathy, Ataxia, And Neuropathy
Research Articles on OPA1
1. increased percentage of apoptotic cells in autosomal dominant optic atrophy patients compared to controls; suggests susceptibility of ADOA cells to oxidative stress and correlation between OPA1 protein dysfunctions and morphological-functional alterations to mitochondria; also results imply sensitivity of mutated protein to free radical damage
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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