Full Product Name
FIGF siRNA (Rat)
Product Synonym Names
VEGFD; Vascular endothelial growth factor D; VEGF-D; c-Fos-induced growth factor; FIGF
Product Gene Name
FIGF sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for O35251
Specificity
FIGF siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat FIGF gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of FIGF sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
FIGF sirna
siRNA to inhibit FIGF expression using RNA interference
Applications Tested/Suitable for FIGF sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for FIGF. It may not necessarily be applicable to this product.
NCBI Accession #
NP_113949.1
[Other Products]
NCBI GenBank Nucleotide #
NM_031761.1
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UniProt Primary Accession #
O35251
[Other Products]
UniProt Secondary Accession #
Q91ZE4[Other Products]
UniProt Related Accession #
O35251[Other Products]
Molecular Weight
37,113 Da
NCBI Official Full Name
vascular endothelial growth factor D
NCBI Official Synonym Full Names
c-fos induced growth factor
NCBI Official Symbol
Figf??[Similar Products]
NCBI Official Synonym Symbols
Vegf-d
??[Similar Products]
NCBI Protein Information
vascular endothelial growth factor D
UniProt Protein Name
Vascular endothelial growth factor D
UniProt Synonym Protein Names
c-Fos-induced growth factor; FIGF
UniProt Gene Name
Figf??[Similar Products]
UniProt Synonym Gene Names
Vegfd; VEGF-D; FIGF??[Similar Products]
UniProt Entry Name
VEGFD_RAT
NCBI Summary for FIGF
binds to and activates the tyrosine kinase receptor VEGFR-3 and VEGFR-2; involved in positive regulation of lymphatic blood vessel growth [RGD, Feb 2006]
UniProt Comments for FIGF
Growth factor active in angiogenesis, lymphangiogenesis and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in the formation of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in *****s. Binds and activates VEGFR-3 (Flt4) receptor ().
Research Articles on FIGF
1. Expression of VEGF-D and its receptor, VEGFR-3, are enhanced in various cell types in the infarcted heart.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
