Product Name
TRPA1, siRNA
Full Product Name
TRPA1 siRNA (Rat)
Product Synonym Names
ANKTM1; Transient receptor potential cation channel subfamily A member 1; Ankyrin-like with transmembrane domains protein 1
Product Gene Name
TRPA1 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q6RI86
Specificity
TRPA1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat TRPA1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of TRPA1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
TRPA1 sirna
siRNA to inhibit TRPA1 expression using RNA interference
Applications Tested/Suitable for TRPA1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for TRPA1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_997491.1
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NCBI GenBank Nucleotide #
NM_207608.1
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UniProt Primary Accession #
Q6RI86
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UniProt Related Accession #
Q6RI86[Other Products]
Molecular Weight
128,602 Da
NCBI Official Full Name
transient receptor potential cation channel subfamily A member 1
NCBI Official Synonym Full Names
transient receptor potential cation channel, subfamily A, member 1
NCBI Official Symbol
Trpa1??[Similar Products]
NCBI Official Synonym Symbols
Anktm1
??[Similar Products]
NCBI Protein Information
transient receptor potential cation channel subfamily A member 1
UniProt Protein Name
Transient receptor potential cation channel subfamily A member 1
UniProt Synonym Protein Names
Ankyrin-like with transmembrane domains protein 1
Protein Family
Transient receptor potential cation channel subfamily
UniProt Gene Name
Trpa1??[Similar Products]
UniProt Synonym Gene Names
Anktm1??[Similar Products]
UniProt Entry Name
TRPA1_RAT
UniProt Comments for TRPA1
Receptor-activated non-selective cation channel involved in detection of pain and possibly also in cold perception and inner ear function. Has a central role in the pain response to endogenous inflammatory mediators and to a diverse array of volatile irritants, such as mustard oil, cinnamaldehyde, garlic and acrolein, an irritant from tears gas and vehicule exhaust fumes. Acts also as a ionotropic cannabinoid receptor by being activated by delta(9)-tetrahydrocannabinol (THC), the psychoactive component of marijuana (PubMed:14712238). Is also activated by menthol (in vitro) (). May be a component for the mechanosensitive transduction channel of hair cells in inner ear, thereby participating in the perception of sounds. Probably operated by a phosphatidylinositol second messenger system ().
Research Articles on TRPA1
1. TRPA1 is a major mediator of the proinflammatory/proalgesic actions of aromatase inhibitors
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
