Full Product Name
OPA1 siRNA (Rat)
Product Synonym Names
Dynamin-like 120 kDa protein mitochondrial; Optic atrophy protein 1 homolog
Product Gene Name
OPA1 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q2TA68
Specificity
OPA1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat OPA1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of OPA1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
OPA1 sirna
siRNA to inhibit OPA1 expression using RNA interference
Applications Tested/Suitable for OPA1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for OPA1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_598269.3
[Other Products]
NCBI GenBank Nucleotide #
NM_133585.3
[Other Products]
UniProt Primary Accession #
Q2TA68
[Other Products]
UniProt Secondary Accession #
O08681; Q5MPP1; Q5MPP2; Q5QJE9; Q6B435; Q6R611[Other Products]
UniProt Related Accession #
Q2TA68[Other Products]
Molecular Weight
115,561 Da
NCBI Official Full Name
dynamin-like 120 kDa protein, mitochondrial
NCBI Official Synonym Full Names
optic atrophy 1
NCBI Official Symbol
Opa1??[Similar Products]
NCBI Protein Information
dynamin-like 120 kDa protein, mitochondrial
UniProt Protein Name
Dynamin-like 120 kDa protein, mitochondrial
UniProt Synonym Protein Names
Optic atrophy protein 1 homolog
Protein Family
Dynamin-like 120 kDa protein
UniProt Gene Name
Opa1??[Similar Products]
UniProt Entry Name
OPA1_RAT
UniProt Comments for OPA1
OPA1: a dynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space. Defects in OPA1 are the cause of optic atrophy 1 and deafness. Eight splice-variant isoforms have been described.
Protein type: Mitochondrial; Membrane protein, integral; Apoptosis; EC 3.6.5.5; Hydrolase
Cellular Component: nucleoplasm; mitochondrial crista; mitochondrial outer membrane; membrane; mitochondrion; mitochondrial inner membrane; dendrite; cytoplasm; integral to membrane; mitochondrial intermembrane space
Molecular Function: GTPase activity; GTP binding
Biological Process: inner mitochondrial membrane organization and biogenesis; positive regulation of neuron maturation; mitochondrion organization and biogenesis; mitochondrial genome maintenance; visual perception; mitochondrial fusion; metabolic process; apoptosis; neural tube closure; aging
Research Articles on OPA1
1. Data indicate that the expression of the dynamin-related GTPase OPA1 increased significantly in the pretreated groups, especially in the pre-exercised ischemia group compared with the non-treated groups.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
