5-hydroxytryptamine receptor 1B; 5-HT-1B; 5-HT1B; Serotonin receptor 1B; Htr1b; 5ht1b

For Research Use Only. Not for use in diagnostic procedures.

This assay recognizes recombinant and natural mouse 5-HT1B. No significant cross-reactivity or interference was observed.

For long term storage, please store the entire kit at -20 degree C.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of Htr1b elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS289883 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 5-hydroxytryptamine receptor 1B (Htr1b) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Htr1b. The ELISA analytical biochemical technique of the MBS289883 kit is based on Htr1b antibody-Htr1b antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Htr1b antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Htr1b. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of mouse 5-HT1B concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to 5-HT1B. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for 5-HT1B and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain 5-HT1B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of 5-HT1B in the samples is then determined by comparing the O.D. of the samples to the standard curve.

43,079 Da

5-hydroxytryptamine (serotonin) receptor 1B

5-hydroxytryptamine receptor 1B

5-hydroxytryptamine receptor 1B

5ht1b; 5-HT-1B; 5-HT1B??[Similar Products]

5HT1B_MOUSE

5-HT(1B): This is one of the several different receptors for 5- hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity. Belongs to the G-protein coupled receptor 1 family.

Protein type: Receptor, GPCR; Membrane protein, multi-pass; Membrane protein, integral; GPCR, family 1

Cellular Component: cytoplasm; integral to membrane; integral to plasma membrane; membrane; plasma membrane

Molecular Function: drug binding; G-protein coupled receptor activity; serotonin binding; serotonin receptor activity; signal transducer activity

Biological Process: behavior; bone remodeling; drinking behavior; feeding behavior; G-protein coupled receptor internalization; G-protein coupled receptor protein signaling pathway; negative regulation of cAMP biosynthetic process; negative regulation of serotonin secretion; negative regulation of synaptic transmission, GABAergic; negative regulation of synaptic transmission, glutamatergic; protein kinase C activation; regulation of behavior; regulation of dopamine secretion; response to cocaine; response to drug; response to ethanol; response to toxin; serotonin receptor, adenylate cyclase inhibiting pathway; signal transduction; synaptic transmission; vasoconstriction

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.