For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of Human AVP. No significant cross-reactivity or interference between Human AVP and analogues was observed.

Store at 2-8 degree C.

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Small volumes of ADH/VP/AVP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS280869 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Antidiuretic hormone/vasopressin/arginine vasopressin (ADH/VP/AVP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ADH/VP/AVP. The ELISA analytical biochemical technique of the MBS280869 kit is based on ADH/VP/AVP antibody-ADH/VP/AVP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ADH/VP/AVP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ADH/VP/AVP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate AVP in samples. An antibody specific for AVP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAVP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AVP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AVP bound in the initial step. The color development is stopped and the intensity of the color is measured.

17,325 Da

arginine vasopressin

vasopressin-neurophysin 2-copeptin

Vasopressin-neurophysin 2-copeptin

ARVP; VP??[Similar Products]

This gene encodes a member of the vasopressin/oxytocin family and preproprotein that is proteolytically processed to generate multiple protein products. These products include the neuropeptide hormone arginine vasopressin, and two other peptides, neurophysin 2 and copeptin. Arginine vasopressin is a posterior pituitary hormone that is synthesized in the supraoptic nucleus and paraventricular nucleus of the hypothalamus. Along with its carrier protein, neurophysin 2, it is packaged into neurosecretory vesicles and transported axonally to the nerve endings in the neurohypophysis where it is either stored or secreted into the bloodstream. The precursor is thought to be activated while it is being transported along the axon to the posterior pituitary. Arginine vasopressin acts as a growth factor by enhancing pH regulation through acid-base transport systems. It has a direct antidiuretic action on the kidney, and also causes vasoconstriction of the peripheral vessels. This hormone can contract smooth muscle during parturition and lactation. It is also involved in cognition, tolerance, adaptation and complex sexual and maternal behaviour, as well as in the regulation of water excretion and cardiovascular functions. Mutations in this gene cause autosomal dominant neurohypophyseal diabetes insipidus (ADNDI). This gene is present in a gene cluster with the related gene oxytocin on chromosome 20. [provided by RefSeq, Nov 2015]

AVP: Neurophysin 2 specifically binds vasopressin. Defects in AVP are the cause of diabetes insipidus, neurohypophyseal (NDI). A disease characterized by persistent thirst, polydipsia and polyuria. Affected individuals are apparently normal at birth, but characteristically develop symptoms of vasopression deficiency during childhood. Belongs to the vasopressin/oxytocin family.

Protein type: Hormone; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 20p13

Cellular Component: clathrin-coated vesicle membrane; cytosol; dendrite; extracellular region; extracellular space; secretory granule

Molecular Function: caspase inhibitor activity; neurohypophyseal hormone activity; neuropeptide hormone activity; protein kinase activity; receptor binding; signal transducer activity; V1A vasopressin receptor binding; V1B vasopressin receptor binding

Biological Process: apoptosis; cell-cell signaling; elevation of cytosolic calcium ion concentration; generation of precursor metabolites and energy; grooming behavior; hyperosmotic salinity response; locomotory behavior; maternal behavior; negative regulation of apoptosis; negative regulation of cysteine-type endopeptidase activity involved in apoptotic process; negative regulation of female receptivity; negative regulation of transmission of nerve impulse; penile erection; positive regulation of cAMP biosynthetic process; positive regulation of cell growth; positive regulation of cell proliferation; positive regulation of cellular pH reduction; positive regulation of glutamate secretion; positive regulation of peptidyl-serine phosphorylation; positive regulation of prostaglandin biosynthetic process; positive regulation of systemic arterial blood pressure; positive regulation of vasoconstriction; renal water homeostasis; response to ethanol; response to nicotine; response to testosterone stimulus; signal transduction; social behavior; sodium-independent organic anion transport; vasoconstriction; water transport

Disease: Diabetes Insipidus, Neurohypophyseal

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