Full Product Name
MYH9 siRNA (Human)
Product Synonym Names
Myosin-9; Cellular myosin heavy chain. type A; Myosin heavy chain 9; Myosin heavy chain. non-muscle IIa; Non-muscle myosin heavy chain A; NMMHC-A; Non-muscle myosin heavy chain IIa; NMMHC II-a; NMMHC-IIA
Product Gene Name
MYH9 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P35579
Specificity
MYH9 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human MYH9 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of MYH9 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
MYH9 sirna
siRNA to inhibit MYH9 expression using RNA interference
Applications Tested/Suitable for MYH9 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for MYH9. It may not necessarily be applicable to this product.
NCBI Accession #
NP_002464.1
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NCBI GenBank Nucleotide #
NM_002473.5
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UniProt Primary Accession #
P35579
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UniProt Secondary Accession #
O60805; Q60FE2; Q86T83; A8K6E4[Other Products]
UniProt Related Accession #
P35579[Other Products]
Molecular Weight
159,864 Da
NCBI Official Full Name
myosin-9
NCBI Official Synonym Full Names
myosin, heavy chain 9, non-muscle
NCBI Official Symbol
MYH9??[Similar Products]
NCBI Official Synonym Symbols
MHA; FTNS; EPSTS; BDPLT6; DFNA17; NMMHCA; NMHC-II-A; NMMHC-IIA
??[Similar Products]
NCBI Protein Information
myosin-9
UniProt Protein Name
Myosin-9
UniProt Synonym Protein Names
Cellular myosin heavy chain, type A; Myosin heavy chain 9; Myosin heavy chain, non-muscle IIa; Non-muscle myosin heavy chain A; NMMHC-A; Non-muscle myosin heavy chain IIa; NMMHC II-a; NMMHC-IIA
UniProt Gene Name
MYH9??[Similar Products]
UniProt Synonym Gene Names
NMMHC-A; NMMHC II-a; NMMHC-IIA??[Similar Products]
UniProt Entry Name
MYH9_HUMAN
NCBI Summary for MYH9
This gene encodes a conventional non-muscle myosin; this protein should not be confused with the unconventional myosin-9a or 9b (MYO9A or MYO9B). The encoded protein is a myosin IIA heavy chain that contains an IQ domain and a myosin head-like domain which is involved in several important functions, including cytokinesis, cell motility and maintenance of cell shape. Defects in this gene have been associated with non-syndromic sensorineural deafness autosomal dominant type 17, Epstein syndrome, Alport syndrome with macrothrombocytopenia, Sebastian syndrome, Fechtner syndrome and macrothrombocytopenia with progressive sensorineural deafness. [provided by RefSeq, Dec 2011]
UniProt Comments for MYH9
MYH9: Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Interacts with PDLIM2. Interacts with SLC6A4. Myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2). Interacts with RASIP1. Interacts with DDR1. Interacts with SVIL and HTRA3. In the kidney, expressed in the glomeruli. Also expressed in leukocytes. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Motor; Motility/polarity/chemotaxis; Actin-binding
Chromosomal Location of Human Ortholog: 22q13.1
Cellular Component: cortical cytoskeleton; signalosome; protein complex; myosin II complex; leading edge; contractile ring; immunological synapse; actomyosin; cytosol; actin cytoskeleton; ruffle; cell-cell adherens junction; membrane; cytoplasm; plasma membrane; stress fiber; spindle; uropod; nucleus; integrin complex; neuromuscular junction; cleavage furrow
Molecular Function: microfilament motor activity; actin filament binding; calmodulin binding; protein binding; protein homodimerization activity; protein anchor; ATPase activity; motor activity; actin-dependent ATPase activity; ADP binding; actin binding; ATP binding
Biological Process: integrin-mediated signaling pathway; axon guidance; blood vessel endothelial cell migration; monocyte differentiation; actin filament-based movement; in utero embryonic development; membrane protein ectodomain proteolysis; actomyosin structure organization and biogenesis; cytokinesis; uropod organization and biogenesis; establishment of meiotic spindle localization; regulation of cell shape; protein transport; actin cytoskeleton reorganization; ephrin receptor signaling pathway; establishment of T cell polarity; angiogenesis; platelet formation; leukocyte migration; myoblast fusion; meiotic spindle organization and biogenesis
Disease: Deafness, Autosomal Dominant 17; May-hegglin Anomaly; Fechtner Syndrome; Macrothrombocytopenia And Progressive Sensorineural Deafness; Epstein Syndrome; Sebastian Syndrome
Research Articles on MYH9
1. the role of myosin IIA and its specific kinase, myosin light chain kinase, during Shigella intercellular spreading was investigated in HeLa cells
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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