For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

Affinity Purified

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-MYH9 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

This is a rabbit polyclonal antibody against MYH9. It was validated on Western Blot using a cell lysate as a positive control.

Target Description: MYH9 is a myosin IIA heavy chain that contains an IQ domain and a myosin head-like domain. The protein is involved in several important functions, including cytokinesis, cell motility and maintenance of cell shape. Defects in MYH9 are the cause of non-syndromic sensorineural deafness autosomal dominant type 17, Epstein syndrome, Alport syndrome with macrothrombocytopenia, Sebastian syndrome, Fechtner syndrome and macrothrombocytopenia with progressive sensorineural deafness.

Polyclonal; Transcription Factor; Signal Proteins; Chromatin & Nuclear Signaling; Membrane & Traffic; Disease Related; DNA/RNA/Protein Interactions; GPCR; Cell Morphogenesis;

Western Blot (WB)

MYH9 antibody - middle region validated by WB using Hek 293 Whole Cell Lysate at 1:4,000.

WB Suggested Anti-MYH9 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:2500
Positive Control: MCF7 cell lysate

226kDa

myosin heavy chain 9

myosin-9

Myosin-9

NMMHC-A; NMMHC II-a; NMMHC-IIA??[Similar Products]

MYH9_HUMAN

This gene encodes a conventional non-muscle myosin; this protein should not be confused with the unconventional myosin-9a or 9b (MYO9A or MYO9B). The encoded protein is a myosin IIA heavy chain that contains an IQ domain and a myosin head-like domain which is involved in several important functions, including cytokinesis, cell motility and maintenance of cell shape. Defects in this gene have been associated with non-syndromic sensorineural deafness autosomal dominant type 17, Epstein syndrome, Alport syndrome with macrothrombocytopenia, Sebastian syndrome, Fechtner syndrome and macrothrombocytopenia with progressive sensorineural deafness. [provided by RefSeq, Dec 2011]

MYH9: Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Interacts with PDLIM2. Interacts with SLC6A4. Myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2). Interacts with RASIP1. Interacts with DDR1. Interacts with SVIL and HTRA3. In the kidney, expressed in the glomeruli. Also expressed in leukocytes. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motor; Motility/polarity/chemotaxis; Actin-binding

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: cortical cytoskeleton; signalosome; protein complex; myosin II complex; leading edge; contractile ring; immunological synapse; actomyosin; cytosol; actin cytoskeleton; ruffle; cell-cell adherens junction; membrane; cytoplasm; plasma membrane; stress fiber; spindle; uropod; integrin complex; neuromuscular junction; nucleus; cleavage furrow

Molecular Function: actin filament binding; calmodulin binding; microfilament motor activity; protein binding; protein homodimerization activity; protein anchor; ATPase activity; motor activity; actin binding; ADP binding; actin-dependent ATPase activity; ATP binding

Biological Process: integrin-mediated signaling pathway; axon guidance; blood vessel endothelial cell migration; monocyte differentiation; actin filament-based movement; in utero embryonic development; actomyosin structure organization and biogenesis; membrane protein ectodomain proteolysis; cytokinesis; uropod organization and biogenesis; regulation of cell shape; establishment of meiotic spindle localization; protein transport; actin cytoskeleton reorganization; ephrin receptor signaling pathway; establishment of T cell polarity; angiogenesis; platelet formation; leukocyte migration; myoblast fusion; meiotic spindle organization and biogenesis

Disease: Deafness, Autosomal Dominant 17; May-hegglin Anomaly; Fechtner Syndrome; Macrothrombocytopenia And Progressive Sensorineural Deafness; Epstein Syndrome; Sebastian Syndrome

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