Product Name
CYP11A1, siRNA
Full Product Name
CYP11A1 siRNA (Human)
Product Synonym Names
CYP11A; Cholesterol side-chain cleavage enzyme mitochondrial; CYPXIA1; Cholesterol desmolase; Cytochrome P450 11A1; Cytochrome P450(scc
Product Gene Name
CYP11A1 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P05108
Specificity
CYP11A1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CYP11A1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of CYP11A1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
CYP11A1 sirna
siRNA to inhibit CYP11A1 expression using RNA interference
Applications Tested/Suitable for CYP11A1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for CYP11A1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000772.2
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NCBI GenBank Nucleotide #
NM_000781.2
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UniProt Primary Accession #
P05108
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UniProt Secondary Accession #
Q15081; Q16805; Q8N1A7; A8K8D5; B3KPU8; G3XAD7[Other Products]
UniProt Related Accession #
P05108[Other Products]
Molecular Weight
42,152 Da
NCBI Official Full Name
cholesterol side-chain cleavage enzyme, mitochondrial isoform a
NCBI Official Synonym Full Names
cytochrome P450, family 11, subfamily A, polypeptide 1
NCBI Official Symbol
CYP11A1??[Similar Products]
NCBI Official Synonym Symbols
CYP11A; CYPXIA1; P450SCC
??[Similar Products]
NCBI Protein Information
cholesterol side-chain cleavage enzyme, mitochondrial
UniProt Protein Name
Cholesterol side-chain cleavage enzyme, mitochondrial
UniProt Synonym Protein Names
CYPXIA1; Cholesterol desmolase; Cytochrome P450 11A1; Cytochrome P450(scc)
Protein Family
Cholesterol side-chain cleavage enzyme
UniProt Gene Name
CYP11A1??[Similar Products]
UniProt Synonym Gene Names
CYP11A??[Similar Products]
UniProt Entry Name
CP11A_HUMAN
NCBI Summary for CYP11A1
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane and catalyzes the conversion of cholesterol to pregnenolone, the first and rate-limiting step in the synthesis of the steroid hormones. Two transcript variants encoding different isoforms have been found for this gene. The cellular location of the smaller isoform is unclear since it lacks the mitochondrial-targeting transit peptide. [provided by RefSeq, Jul 2008]
UniProt Comments for CYP11A1
CYP11A1: Catalyzes the side-chain cleavage reaction of cholesterol to pregnenolone. Defects in CYP11A1 are the cause of adrenal insufficiency congenital with 46,XY sex reversal (AICSR). A rare disorder that can present as acute adrenal insufficiency in infancy or childhood. ACTH and plasma renin activity are elevated and adrenal steroids are inappropriately low or absent; the 46,XY patients have female external genitalia, sometimes with clitoromegaly. The phenotypic spectrum ranges from prematurity, complete underandrogenization, and severe early-onset adrenal failure to term birth with clitoromegaly and later-onset adrenal failure. Patients with congenital adrenal insufficiency do not manifest the massive adrenal enlargement typical of congenital lipoid adrenal hyperplasia. Belongs to the cytochrome P450 family.
Protein type: Mitochondrial; Lipid Metabolism - C21-steroid hormone; Oxidoreductase; EC 1.14.15.6
Chromosomal Location of Human Ortholog: 15q23-q24
Cellular Component: mitochondrial crista; mitochondrion; mitochondrial matrix; perikaryon
Molecular Function: cholesterol monooxygenase (side-chain-cleaving) activity; iron ion binding; heme binding; cholesterol binding
Biological Process: response to alkaloid; steroid metabolic process; maternal process involved in pregnancy; dibenzo-p-dioxin metabolic process; response to insecticide; response to L-ascorbic acid; estrogen biosynthetic process; response to salt stress; response to vitamin E; Leydig cell differentiation; biphenyl metabolic process; cerebellum development; response to gamma radiation; response to corticosterone stimulus; response to drug; cholesterol metabolic process; progesterone biosynthetic process; fractalkine metabolic process; phthalate metabolic process; hippocampus development; mating behavior; vitamin D metabolic process; Schwann cell differentiation; response to genistein; response to hydrogen peroxide; xenobiotic metabolic process; C21-steroid hormone biosynthetic process; sterol metabolic process; granulosa cell differentiation; phenol metabolic process
Disease: Adrenal Insufficiency, Congenital, With 46,xy Sex Reversal, Partial Or Complete
Research Articles on CYP11A1
1. Data indicate a role of cytochrome P450 11A1 (CYP11A1 in sterol metabolism.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
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