ADAMTS9 (A disintegrin and metalloproteinase with thrombospondin motifs 9)/ADAM-TS 9/ADAM-TS9/ADAMTS-9

For Research Use Only. Not for use in diagnostic procedures.

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

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Small volumes of ADAMTS9 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS761193 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A disintegrin and metalloproteinase with thrombospondin motifs 9 (ADAMTS9) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ADAMTS9. The ELISA analytical biochemical technique of the MBS761193 kit is based on ADAMTS9 antibody-ADAMTS9 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ADAMTS9 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ADAMTS9. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human ADAMTS9 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human ADAMTS9 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human ADAMTS9 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofHuman ADAMTS9 can be calculated.

213,420 Da

ADAM metallopeptidase with thrombospondin type 1 motif 9

A disintegrin and metalloproteinase with thrombospondin motifs 9

A disintegrin and metalloproteinase with thrombospondin motifs 9

KIAA1312; ADAM-TS 9; ADAM-TS9; ADAMTS-9??[Similar Products]

ATS9_HUMAN

This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. Members of the ADAMTS family have been implicated in the cleavage of proteoglycans, the control of organ shape during development, and the inhibition of angiogenesis. This gene is localized to chromosome 3p14.3-p14.2, an area known to be lost in hereditary renal tumors. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing. [provided by RefSeq, Jan 2016]

ADAMTS9: Cleaves the large aggregating proteoglycans, aggrecan and versican. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide; EC 3.4.24.-; Extracellular matrix; Motility/polarity/chemotaxis; Protease

Chromosomal Location of Human Ortholog: 3p14.1

Cellular Component: endoplasmic reticulum; extracellular matrix; proteinaceous extracellular matrix

Molecular Function: metalloendopeptidase activity; metallopeptidase activity; zinc ion binding

Biological Process: glycoprotein catabolic process; multicellular organismal development; protein transport; proteolysis; vesicle-mediated transport

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