Product Name
TECPR2, siRNA
Full Product Name
TECPR2 siRNA (Human)
Product Synonym Names
KIAA0297; KIAA0329; Tectonin beta-propeller repeat-containing protein 2; WD repeat-containing protein KIAA0329/KIAA0297
Product Gene Name
TECPR2 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for O15040
Specificity
TECPR2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human TECPR2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of TECPR2 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
TECPR2 sirna
siRNA to inhibit TECPR2 expression using RNA interference
Applications Tested/Suitable for TECPR2 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for TECPR2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001166102.1
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NCBI GenBank Nucleotide #
NM_001172631.2
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UniProt Primary Accession #
O15040
[Other Products]
UniProt Secondary Accession #
Q9UEG6; A5PKY3; A6NFY9; A7E2X3; H0YMM9[Other Products]
UniProt Related Accession #
O15040[Other Products]
Molecular Weight
137,944 Da
NCBI Official Full Name
tectonin beta-propeller repeat-containing protein 2 isoform 2
NCBI Official Synonym Full Names
tectonin beta-propeller repeat containing 2
NCBI Official Symbol
TECPR2??[Similar Products]
NCBI Official Synonym Symbols
SPG49; KIAA0329
??[Similar Products]
NCBI Protein Information
tectonin beta-propeller repeat-containing protein 2
UniProt Protein Name
Tectonin beta-propeller repeat-containing protein 2
UniProt Synonym Protein Names
WD repeat-containing protein KIAA0329/KIAA0297
Protein Family
Tectonin beta-propeller repeat-containing protein
UniProt Gene Name
TECPR2??[Similar Products]
UniProt Synonym Gene Names
KIAA0297; KIAA0329??[Similar Products]
UniProt Entry Name
TCPR2_HUMAN
NCBI Summary for TECPR2
The protein encoded by this gene is a member of the tectonin beta-propeller repeat-containing (TECPR) family, and contains both TECPR and tryptophan-aspartic acid repeat (WD repeat) domains. This gene has been implicated in autophagy, as reduced expression levels of this gene have been associated with impaired autophagy. Recessive mutations in this gene have been associated with a hereditary form of spastic paraparesis (HSP). HSP is characterized by progressive spasticity and paralysis of the legs. There is also some evidence linking mutations in this gene with birdshot chorioretinopathy (BSCR), which results in inflammation of the choroid and retina. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2015]
UniProt Comments for TECPR2
TECPR2: Belongs to the WD repeat KIAA0329 family.
Protein type: Unknown function
Chromosomal Location of Human Ortholog: 14q32.31
Molecular Function: protein binding
Biological Process: autophagy
Disease: Spastic Paraplegia 49, Autosomal Recessive
Research Articles on TECPR2
1. The discovered TECPR2 mutation implicates autophagy, a central intracellular mechanism, in spastic paraparesis.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
