Product Name
SLC36A2, siRNA
Full Product Name
SLC36A2 siRNA (Human)
Product Synonym Names
PAT2; TRAMD1; Proton-coupled amino acid transporter 2; Proton/amino acid transporter 2; Solute carrier family 36 member 2; Tramdorin-1
Product Gene Name
SLC36A2 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q495M3
Specificity
SLC36A2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SLC36A2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of SLC36A2 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
SLC36A2 sirna
siRNA to inhibit SLC36A2 expression using RNA interference
Applications Tested/Suitable for SLC36A2 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for SLC36A2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_861441.2
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NCBI GenBank Nucleotide #
NM_181776.2
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UniProt Primary Accession #
Q495M3
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UniProt Secondary Accession #
Q495M4; Q495M6; Q6ZWK5; Q7Z6B5[Other Products]
UniProt Related Accession #
Q495M3[Other Products]
Molecular Weight
22,680 Da
NCBI Official Full Name
proton-coupled amino acid transporter 2
NCBI Official Synonym Full Names
solute carrier family 36 (proton/amino acid symporter), member 2
NCBI Official Symbol
SLC36A2??[Similar Products]
NCBI Official Synonym Symbols
PAT2; TRAMD1
??[Similar Products]
NCBI Protein Information
proton-coupled amino acid transporter 2
UniProt Protein Name
Proton-coupled amino acid transporter 2
UniProt Synonym Protein Names
Solute carrier family 36 member 2; Tramdorin-1
Protein Family
Proton-coupled amino acid transporter
UniProt Gene Name
SLC36A2??[Similar Products]
UniProt Synonym Gene Names
PAT2; TRAMD1; Proton/amino acid transporter 2??[Similar Products]
UniProt Entry Name
S36A2_HUMAN
NCBI Summary for SLC36A2
This gene encodes a pH-dependent proton-coupled amino acid transporter that belongs to the amino acid auxin permease 1 protein family. The encoded protein primarily transports small amino acids such as glycine, alanine and proline. Mutations in this gene are associated with iminoglycinuria and hyperglycinuria. [provided by RefSeq, Sep 2010]
UniProt Comments for SLC36A2
SLC36A2: Involved in a pH-dependent electrogenic neuronal transport and sequestration of small amino acids. Transports glycine and proline. Inhibited by sarcosine. Defects in SLC36A2 are a cause of hyperglycinuria (HG). It is a condition characterized by excess of glycine in the urine. In some cases it is associated with renal colic and renal oxalate stones. Defects in SLC36A2 are a cause of iminoglycinuria (IG). It is a disorder of renal tubular reabsorption of glycine and imino acids (proline and hydroxyproline), marked by excessive levels of all three substances in the urine. Mutations in SLC36A2 that retain residual transport activity result in the IG phenotype only when combined with haploinsufficiency of the imino acid transporter SLC6A20 or deficiency of the neutral amino acid transporter SLC6A19. Additional polymorphisms and mutations in SLC6A18 can contribute to iminoglycinuria in some families. Belongs to the amino acid/polyamine transporter 2 family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Transporter; Membrane protein, multi-pass; Transporter, SLC family; Membrane protein, integral
Chromosomal Location of Human Ortholog: 5q33.1
Cellular Component: vacuolar membrane; integral to membrane; plasma membrane
Molecular Function: glycine transmembrane transporter activity; L-proline transmembrane transporter activity; hydrogen ion transmembrane transporter activity; hydrogen:amino acid symporter activity; L-alanine transmembrane transporter activity
Biological Process: glycine transport; proton transport; amino acid transport; ion transport; transmembrane transport; L-alanine transport
Disease: Iminoglycinuria; Hyperglycinuria
Research Articles on SLC36A2
1. Observational study of gene-disease association. (HuGE Navigator)
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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