Platelet Factor 4, Purified (Clone: RTO) (Mouse IgG2b); Anti-Human Platelet Factor IV

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 4; NC_000004.11 (74719013..74720198). Location: 4q12-q21

Monoclonal

Mouse IgG2bkappa

RTO

Mouse

Platelet Factor 4 (This antibody is specific for Human Platelet Factor IV.)

Purified

1.0 mg/ml (lot specific)

Store at +4 degree C. For long term storage, aliquot and freeze unused portion at -20 degree C in volumes appropriate for single use. Avoid freeze thaw cycles.

Small volumes of anti-PF4V1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Platelet factor IV is a 70 aa protein released from the alpha granules of activated platelets. PF4 is synthesized by bone marrow megakaryocytes and stored in alpha granules as a non-covalent bound tetramer. Platelet factor IV binds with high affinity to heparin and plays a role in inflammation and wound repair. PF4 is a chemoattractant for neutrophils, monocytes and fibroblasts and has been reported to be an immunologic regulator that inhibits suppressor T-cell activity.

MBS520501 (clone RTO) is seen to bind free PF4 in the absence of any bound heparin. This is unlike MBS520552 (clone KKO), which binds PF4/heparin complexes to a similar degree as auto-antibodies found in individuals with Heparin-Induced Thrombocytopenia/Thrombosis (HIT/HITT).

ELISA (EIA), Western Blot (WB)

This antibody is suitable for use in ELISA.
ELISA: Use at a concentration of 0.016ug/ml

platelet factor 4 variant 1

platelet factor 4 variant; PF4alt; PF4var1; C-X-C motif chemokine 4; platelet factor 4, variant 1 (PF4-like)

Platelet factor 4 variant

CXCL4V1; SCYB4V1??[Similar Products]

PF4V_HUMAN

Function: Inhibitor of angiogenesis. Inhibitor of endothelial cell chemotaxis (in vitro). Ref.5

Subunit structure: Homotetramer. Ref.6

Subcellular location: Secreted.

Post-translational modification: The N-terminal processed forms of platelet factor 4 variant seems to be produced by proteolytic cleavage. The most abundant form is Platelet factor 4 variant(5-74).

Miscellaneous: Binding to heparin is much weaker than in the close homolog PF4/CXCL4.

Sequence similarities: Belongs to the intercrine alpha (chemokine CxC) family.

Mass spectrometry: Molecular mass is 8250.9 Da from positions 31 - 104. Determined by ESI. Platelet factor 4 variant. Ref.5Molecular mass is 7877.7 Da from positions 34 - 104. Determined by ESI. Platelet factor 4 variant(4-74). Ref.5Molecular mass is 7805.8 Da from positions 35 - 104. Determined by ESI. Platelet factor 4 variant(5-74). Ref.5Molecular mass is 7678.6 Da from positions 36 - 104. Determined by ESI. Platelet factor 4 variant(6-74). Ref.5

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