Product Name
Platelet Factor 4 (PF4V1), Monoclonal Antibody
Full Product Name
Anti-Human Platelet Factor 4 (requires heparin for binding), Biotin (Clone: KKO) (Mouse IgG2b)
Product Synonym Names
Platelet Factor 4 (requires heparin for binding), Biotin (Clone: KKO) (Mouse IgG2b); Anti-Human Platelet Factor IV/Heparin
Product Gene Name
anti-PF4V1 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 4; NC_000004.11 (74719013..74720198). Location: 4q12-q21
3D Structure
ModBase 3D Structure for P10720
Specificity
Platelet Factor 4 (requires heparin for binding) (This antibody is specific for Human Platelet Factor IV/heparin complexes)
Concentration
0.1 mg/ml (lot specific)
Immunogen
Human Factor IV purified from the supernatant of thrombin activated platelets.
Presentation
Biotin conjugated IgG buffered in PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL.
Preparation and Storage
Store at +4 degree C. For long term storage, aliquot and freeze unused portion at -20 degree C in volumes appropriate for single use. Avoid freeze thaw cycles.
Other Notes
Small volumes of anti-PF4V1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-PF4V1 antibody
Platelet factor IV is a 70 aa protein released from the alpha granules of activated platelets. PF4 is synthesized by bone marrow megakaryocytes and stored in alpha granules as a non-covalent bound tetramer. Platelet factor IV binds with high affinity to heparin and plays a role in inflammation and wound repair. PF4 is a chemoattractant for neutrophils, monocytes and fibroblasts and has been reported to be an immunologic regulator that inhibits suppressor T-cell activity. Heparin-Induced Thrombocytopenia/Thrombosis (HIT/HITT) is a life-threatening complication that manifests itself in a small population of patients exposed to intravenous-heparin. It is characterized by the production of PF4/heparin auto-antibodies. These auto-antibodies bind the PF4/heparin complexes and subsequently bind the FcgammaIIa receptor on the platelets surface through their Fc region. This activates the platelets and can initiate clot formation. (clone KKO), unlike (clone RTO) which binds PF4 alone, is specific for PF4/heparin complexes and is seen to have similar binding properties as auto-antibodies found in HIT/HITT.
Applications Tested/Suitable for anti-PF4V1 antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-PF4V1 antibody
This antibody is suitable for use in ELISA and Western Blot
NCBI/Uniprot data below describe general gene information for PF4V1. It may not necessarily be applicable to this product.
NCBI Accession #
AAA60067.1
[Other Products]
UniProt Primary Accession #
P10720
[Other Products]
UniProt Secondary Accession #
A1L4S0[Other Products]
UniProt Related Accession #
P10720[Other Products]
Molecular Weight
11,553 Da[Similar Products]
NCBI Official Full Name
platelet factor 4
NCBI Official Synonym Full Names
platelet factor 4 variant 1
NCBI Official Symbol
PF4V1??[Similar Products]
NCBI Official Synonym Symbols
PF4A; CXCL4L1; CXCL4V1; PF4-ALT; SCYB4V1
??[Similar Products]
NCBI Protein Information
platelet factor 4 variant; PF4alt; PF4var1; C-X-C motif chemokine 4; platelet factor 4, variant 1 (PF4-like)
UniProt Protein Name
Platelet factor 4 variant
UniProt Synonym Protein Names
C-X-C motif chemokine 4 variant; CXCL4L1; PF4alt; PF4var1
Protein Family
Platelet factor
UniProt Gene Name
PF4V1??[Similar Products]
UniProt Synonym Gene Names
CXCL4V1; SCYB4V1??[Similar Products]
UniProt Entry Name
PF4V_HUMAN
UniProt Comments for PF4V1
Function: Inhibitor of angiogenesis. Inhibitor of endothelial cell chemotaxis (in vitro). Ref.5
Subunit structure: Homotetramer. Ref.6
Subcellular location: Secreted.
Post-translational modification: The N-terminal processed forms of platelet factor 4 variant seems to be produced by proteolytic cleavage. The most abundant form is Platelet factor 4 variant(5-74).
Miscellaneous: Binding to heparin is much weaker than in the close homolog PF4/CXCL4.
Sequence similarities: Belongs to the intercrine alpha (chemokine CxC) family.
Mass spectrometry: Molecular mass is 8250.9 Da from positions 31 - 104. Determined by ESI. Platelet factor 4 variant. Ref.5Molecular mass is 7877.7 Da from positions 34 - 104. Determined by ESI. Platelet factor 4 variant(4-74). Ref.5Molecular mass is 7805.8 Da from positions 35 - 104. Determined by ESI. Platelet factor 4 variant(5-74). Ref.5Molecular mass is 7678.6 Da from positions 36 - 104. Determined by ESI. Platelet factor 4 variant(6-74). Ref.5
Research Articles on PF4V1
1. Structural insight into the novel functional properties of human CXCL4L1.
Precautions
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Disclaimer
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