Full Product Name
Mouse Monoclonal [clone 7C9] (IgG1) to Human MYL2
Product Synonym Names
Anti-MYL2 Antibody (clone 7C9) IHC-plus; MYL2; MLC2; Myosin light chain 2; MLC-2; RLC of myosin; CMH10; MLC-2v; Human MYL2
Product Gene Name
anti-MYL2 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P10916
Purity/Purification
Ascites
Form/Format
Ascites fluid, 0.03% sodium azide
Immunogen Description
Purified recombinant fragment of MYL2 expressed in E Coli.
Immunogen
MYL2 antibody was raised against purified recombinant fragment of MYL2 expressed in E Coli.
Preparation and Storage
Long term: -20 degree C; Short term: +4 degree C. Avoid repeat freeze-thaw cycles.
Other Notes
Small volumes of anti-MYL2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-MYL2 antibody
MYL2 encodes the regulatory light chain associated with cardiac myosin beta (or slow) heavy chain. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in MYL2 are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy.
Applications Tested/Suitable for anti-MYL2 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-MYL2 antibody
ELISA (1:10000), IHC-P (1:100), WB (1:500 - 1:2000)
Immunohistochemistry (IHC) of anti-MYL2 antibody
Anti-MYL2 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Western Blot (WB) of anti-MYL2 antibody
Western blot of anti-MYL3 (1) and anti-MYL2 (2) monoclonal antibody against rat fetal heart tissue lysate.
NCBI/Uniprot data below describe general gene information for MYL2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000423.2
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NCBI GenBank Nucleotide #
NM_000432.3
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UniProt Primary Accession #
P10916
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UniProt Secondary Accession #
Q16123[Other Products]
UniProt Related Accession #
P10916[Other Products]
Molecular Weight
18,789 Da
NCBI Official Full Name
myosin regulatory light chain 2, ventricular/cardiac muscle isoform
NCBI Official Synonym Full Names
myosin, light chain 2, regulatory, cardiac, slow
NCBI Official Symbol
MYL2??[Similar Products]
NCBI Official Synonym Symbols
MLC2; CMH10
??[Similar Products]
NCBI Protein Information
myosin regulatory light chain 2, ventricular/cardiac muscle isoform; MLC-2; MLC-2v; RLC of myosin; myosin light chain 2; regulatory light chain of myosin; cardiac ventricular myosin light chain 2; slow cardiac myosin regulatory light chain 2; myosin, light polypeptide 2, regulatory, cardiac, slow
UniProt Protein Name
Myosin regulatory light chain 2, ventricular/cardiac muscle isoform
Protein Family
Myosin regulatory
UniProt Gene Name
MYL2??[Similar Products]
UniProt Synonym Gene Names
MLC-2; MLC-2v??[Similar Products]
UniProt Entry Name
MLRV_HUMAN
NCBI Summary for MYL2
Thus gene encodes the regulatory light chain associated with cardiac myosin beta (or slow) heavy chain. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in this gene are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy. [provided by RefSeq, Jul 2008]
UniProt Comments for MYL2
MRLC2V: myosin regulatory light chain 2, ventricular/cardiac muscle isoform. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in MYL2 are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy.
Protein type: Contractile
Chromosomal Location of Human Ortholog: 12q24.11
Cellular Component: sarcomere; cytoskeleton; myofibril; myosin complex; cytosol; A band; actin cytoskeleton
Molecular Function: actin monomer binding; protein binding; structural constituent of muscle; calcium ion binding; myosin heavy chain binding
Biological Process: muscle cell fate specification; heart contraction; regulation of striated muscle contraction; ventricular cardiac muscle morphogenesis; cardiac myofibril assembly; negative regulation of cell growth; muscle fiber development; post-embryonic development; muscle filament sliding; cardiac muscle contraction
Disease: Cardiomyopathy, Familial Hypertrophic, 10
Research Articles on MYL2
1. Results suggest that Aurora B, but not Rho/MLCK (myosin-light-chain kinase) signaling, is essential for the localization of 2P-MRLC (myosin regulatory light chains) to the midzone in dividing HeLa cells.
Precautions
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Disclaimer
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