ZNF422; MRXSSD; JHDM1F; Jumonji C Domain-Containing Histone Demethylase 1F; Histone lysine demethylase PHF8

For Research Use Only. Not for use in diagnostic procedures.

E Coli

>95%

Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.

Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.

Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customera??s specifications, please inquire.

Small volumes of PHF8 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).

98,290 Da

PHD finger protein 8

histone lysine demethylase PHF8

Histone lysine demethylase PHF8

KIAA1111; ZNF422??[Similar Products]

PHF8_HUMAN

The protein encoded by this gene is a histone lysine demethylase that preferentially acts on histones in the monomethyl or dimethyl states. The encoded protein requires Fe(2+) ion, 2-oxoglutarate, and oxygen for its catalytic activity. Defects in this gene are a cause of mental retardation syndromic X-linked Siderius type (MRXSSD). Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2010]

PHF8: a PHD finger protein with N(epsilon)-methyl lysine histone demethylase activity. Associated with mental retardation and cleft lip/palate. Its active site is closely related to those of FBXL10 and 11, all of which are selective for di- and mono-methylated lysine. In contrast, the JMJD2 demethylases are selective for tri- and di-methylated lysine.

Protein type: Demethylase; Nucleolus; EC 1.14.11.27

Chromosomal Location of Human Ortholog: Xp11.22

Cellular Component: nuclear membrane; nucleolus; nucleoplasm; nucleus

Molecular Function: chromatin binding; histone demethylase activity; histone demethylase activity (H3-K36 specific); histone demethylase activity (H3-K9 specific); iron ion binding; methylated histone residue binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors; protein binding; zinc ion binding

Biological Process: brain development; establishment and/or maintenance of chromatin architecture; G1/S transition of mitotic cell cycle; histone H3-K9 demethylation; mitotic cell cycle; positive regulation of transcription from RNA polymerase I promoter; positive regulation of transcription, DNA-dependent; transcription, DNA-dependent

Disease: Siderius X-linked Mental Retardation Syndrome

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