For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C.

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of ATP2C1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS7221677 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Calcium-transporting ATPase type 2C member 1 (ATP2C1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ATP2C1. The ELISA analytical biochemical technique of the MBS7221677 kit is based on ATP2C1 antibody-ATP2C1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ATP2C1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ATP2C1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Signal Transduction

104,032 Da

ATPase, Ca++ transporting, type 2C, member 1

calcium-transporting ATPase type 2C member 1; HUSSY-28; ATPase 2C1; ATPase, Ca(2+)-sequestering; ATP-dependent Ca(2+) pump PMR1; secretory pathway Ca2+/Mn2+ ATPase 1

Calcium-transporting ATPase type 2C member 1

KIAA1347; PMR1L; HUSSY-28; ATPase 2C1??[Similar Products]

AT2C1_HUMAN

The protein encoded by this gene belongs to the family of P-type cation transport ATPases. This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium ions. Defects in this gene cause Hailey-Hailey disease, an autosomal dominant disorder. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]

ATP2C1: This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of the calcium. Defects in ATP2C1 are the cause of Hailey-Hailey disease (HHD); also known as benign familial pemphigus. HHD is an autosomal dominant disorder characterized by persistent blisters and suprabasal cell separation (acantholysis) of the epidermis, due to impaired keratinocyte adhesion. Patients lacking all isoforms except isoform 2 have HHD. Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter, ion channel; Membrane protein, multi-pass; Hydrolase; Transporter; Membrane protein, integral; EC 3.6.3.8

Chromosomal Location of Human Ortholog: 3q22.1

Cellular Component: Golgi membrane; Golgi apparatus; membrane; integral to membrane; trans-Golgi network

Molecular Function: manganese-transporting ATPase activity; signal transducer activity; calcium-transporting ATPase activity; metal ion binding; manganese ion binding; calcium ion binding; ATP binding

Biological Process: epidermis development; positive regulation of I-kappaB kinase/NF-kappaB cascade; metabolic process; manganese ion transport; signal transduction; cellular calcium ion homeostasis; Golgi calcium ion homeostasis; calcium ion transport; calcium-dependent cell-cell adhesion; actin cytoskeleton reorganization; cellular manganese ion homeostasis; Golgi calcium ion transport; transmembrane transport

Disease: Benign Chronic Pemphigus

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