Purified Rabbit Polyclonal Antibody (Pab); Myosin regulatory light chain 2; ventricular/cardiac muscle isoform; MLC-2; MLC-2v; MYL2

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Ig

Rabbit

This MYL2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 42-75 amino acids from the Central region of human MYL2.

Purified through a protein A column, followed by peptide affinity purification.

Supplied in PBS with 0.09% (W/V) sodium azide.

Maintain refrigerated at 2-8 degree C for up to 2 weeks.
For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-MYL2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Contractile protein that plays a role in heart development and function (By similarity). Following phosphorylation, plays a role in cross-bridge cycling kinetics and cardiac muscle contraction by increasing myosin lever arm stiffness and promoting myosin head diffusion; as a consequence of the increase in maximum contraction force and calcium sensitivity of contraction force. These events altogether slow down myosin kinetics and prolong duty cycle resulting in accumulated myosins being cooperatively recruited to actin s to sustain thin filament activation as a means to fine-tune myofilament calcium sensitivity to force (By similarity). During cardiogenesis plays an early role in cardiac contractility by promoting cardiac myofibril assembly (By similarity).

Primary Antibodies; Cardiovascular; Signal Transduction; Stem Cells

Flow Cytometry (FC/FACS), Western Blot (WB), ELISA (EIA)

FC/FACS: 1:25
WB: 1:2000

Overlay histogram showing U-2 OS cells stained with AP22304c(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22304c, 1:25 dilution) for 60 min at 37 degree C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37 degree C. Isotype control antibody (blue line) was rabbit IgG1 (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

All lanes : Anti-MYL2 Antibody (Center) at 1:2000 dilution
Lane 1: Human heart lysate
Lane 2: Human skeletal muslce lysate
Lane 3: Mouse heart lysate
Lane 4: Rat heart lysate


Lysates/proteins at 20 ug per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 19 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

18789

myosin light chain 2

myosin regulatory light chain 2, ventricular/cardiac muscle isoform

Myosin regulatory light chain 2, ventricular/cardiac muscle isoform

MLC-2s/v??[Similar Products]

Thus gene encodes the regulatory light chain associated with cardiac myosin beta (or slow) heavy chain. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in this gene are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy. [provided by RefSeq, Jul 2008]

Contractile protein that plays a role in heart development and function (). Following phosphorylation, plays a role in cross-bridge cycling kinetics and cardiac muscle contraction by increasing myosin lever arm stiffness and promoting myosin head diffusion; as a consequence of the increase in maximum contraction force and calcium sensitivity of contraction force. These events altogether slow down myosin kinetics and prolong duty cycle resulting in accumulated myosins being cooperatively recruited to actin s to sustain thin filament activation as a means to fine-tune myofilament calcium sensitivity to force (). During cardiogenesis plays an early role in cardiac contractility by promoting cardiac myofibril assembly ().

Libera L.D.,et al.Nucleic Acids Res. 17:2360-2360(1989). Wu Q.L.,et al. Submitted (MAR-1996) to the EMBL/GenBank/DDBJ databases. Margossian S.S.,et al. Submitted (AUG-1997) to the EMBL/GenBank/DDBJ databases. Wadgaonkar R.,et al.Cell. Mol. Biol. Res. 39:13-26(1993). Kovalyov L.I.,et al.Electrophoresis 16:1160-1169(1995).

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.