Product Name
FKHR (FOXO1), Blocking Peptide
Full Product Name
FKHR Antibody (Center D469) Blocking Peptide
Product Synonym Names
Forkhead box protein O1; Forkhead box protein O1A; Forkhead in rhabdomyosarcoma; FOXO1; FKHR; FOXO1A
Product Gene Name
FOXO1 blocking peptide
[Similar Products]
Product Synonym Gene Name
FKHR; FOXO1A[Similar Products]
Antibody/Peptide Pairs
FKHR peptide (MBS9226222) is used for blocking the activity of FKHR antibody (MBS9214571)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q12778
Specificity
The synthetic peptide sequence used to generate the antibody was selected from the Center region of human FKHR. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
Form/Format
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
Cellular Location
Cytoplasm. Nucleus. Note: Shuttles between the cytoplasm and nucleus. Largely nuclear in unstimulated cells. In osteoblasts, colocalizes with ATF4 and RUNX2 in the nucleus (By similarity). Insulin-induced phosphorylation at Ser-256 by PKB/AKT1 leads, via stimulation of Thr-24 phosphorylation, to binding of 14-3-3 proteins and nuclear export to the cytoplasm where it is degraded by the ubiquitin-proteosomal pathway Phosphorylation at Ser-249 by CDK1 disrupts binding of 14-3-3 proteins and promotes nuclear accumulation. Phosphorylation by NLK results in nuclear export. Translocates to the nucleus upon oxidative stress-induced phosphorylation at Ser-212 by STK4/MST1 SGK1-mediated phosphorylation also results in nuclear translocation. Retained in the nucleus under stress stimuli including oxidative stress, nutrient deprivation or nitric oxide Retained in the nucleus on methylation.
Tissue Location
Ubiquitous..
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.
Other Notes
Small volumes of FOXO1 blocking peptide vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
FOXO1 blocking peptide
Transcription factor that is the main target of insulin signaling and regulates metabolic homeostasis in response to oxidative stress. Binds to the insulin response element (IRE) with consensus sequence 5'-TT[G/A]TTTTG-3' and the related Daf-16 family binding element (DBE) with consensus sequence 5'- TT[G/A]TTTAC-3'. Activity suppressed by insulin. Main regulator of redox balance and osteoblast numbers and controls bone mass. Orchestrates the endocrine function of the skeleton in regulating glucose metabolism. Acts synergistically with ATF4 to suppress osteocalcin/BGLAP activity, increasing glucose levels and triggering glucose intolerance and insulin insensitivity. Also suppresses the transcriptional activity of RUNX2, an upstream activator of osteocalcin/BGLAP. In hepatocytes, promotes gluconeogenesis by acting together with PPARGC1A to activate the expression of genes such as IGFBP1, G6PC and PPCK1. Important regulator of cell death acting downstream of CDK1, PKB/AKT1 and SKT4/MST1. Promotes neural cell death. Mediates insulin action on adipose. Regulates the expression of adipogenic genes such as PPARG during preadipocyte differentiation and, adipocyte size and adipose tissue-specific gene expression in response to excessive calorie intake. Regulates the transcriptional activity of GADD45A and repair of nitric oxide-damaged DNA in beta-cells. Required for the autophagic cell death induction in response to starvation or oxidative stress in a transcription-independent manner.
NCBI/Uniprot data below describe general gene information for FOXO1. It may not necessarily be applicable to this product.
NCBI Accession #
Q12778.2
[Other Products]
UniProt Primary Accession #
Q12778
[Other Products]
UniProt Secondary Accession #
O43523; Q5VYC7; Q6NSK6[Other Products]
UniProt Related Accession #
Q12778[Other Products]
Molecular Weight
69,662 Da
NCBI Official Full Name
Forkhead box protein O1
NCBI Official Synonym Full Names
forkhead box O1
NCBI Official Symbol
FOXO1??[Similar Products]
NCBI Official Synonym Symbols
FKH1; FKHR; FOXO1A
??[Similar Products]
NCBI Protein Information
forkhead box protein O1
UniProt Protein Name
Forkhead box protein O1
UniProt Synonym Protein Names
Forkhead box protein O1A; Forkhead in rhabdomyosarcoma
Protein Family
Forkhead box protein
UniProt Gene Name
FOXO1??[Similar Products]
UniProt Synonym Gene Names
FKHR; FOXO1A??[Similar Products]
UniProt Entry Name
FOXO1_HUMAN
NCBI Summary for FOXO1
This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. [provided by RefSeq, Jul 2008]
UniProt Comments for FOXO1
FOXO1A: a transcription factor of the forkhead family. A central regulator of metabolism in several cell types. May play an important role in myogenic growth and differentiation, and in the regulation of lipolysis in adipocytes by controlling the expression of adipose triglyceride lipase (ATGL), the rate-limiting lipolytic enzyme. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. Contains 1 fork-head domain.
Protein type: DNA-binding; Oncoprotein; Transcription factor; Nuclear receptor co-regulator
Chromosomal Location of Human Ortholog: 13q14.1
Cellular Component: cytoplasm; cytosol; mitochondrion; nucleoplasm; nucleus
Molecular Function: beta-catenin binding; chromatin binding; protein binding; protein phosphatase 2A binding; sequence-specific DNA binding; ubiquitin protein ligase binding
Biological Process: cell glucose homeostasis; cellular response to insulin stimulus; cellular response to starvation; endocrine pancreas development; fat cell differentiation; insulin receptor signaling pathway; negative regulation of apoptosis; negative regulation of fat cell differentiation; negative regulation of stress-activated MAPK cascade; negative regulation of transcription, DNA-dependent; positive regulation of apoptosis; positive regulation of autophagy; positive regulation of protein catabolic process; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; protein amino acid acetylation; response to DNA damage stimulus; thermoregulation
Disease: Rhabdomyosarcoma 2
Research Articles on FOXO1
1. Foxo1 and PDX1 are colocalized in fetal pancreatic islets, suggesting they function in parallel in beta cells.
Precautions
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