Aspartate tRNA ligase 2 mitochondrial; Aspartate tRNA ligase; Aspartate tRNA ligase mitochondrial; Aspartate--tRNA ligase; Aspartyl tRNA synthetase 2 (mitochondrial); Aspartyl tRNA synthetase mitochondrial; Aspartyl-tRNA synthetase; AspRS; DARS2; FLJ10514; LBSL, mitochondrial; MT ASPRS; RP3 383J4.2; SYDM_HUMAN

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

DARS2 Antibody detects endogenous levels of DARS2.

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).

Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

1mg/ml (lot specific)

Store at -20 degree C. Stable for 12 months from date of receipt.

Manufactured in an ISO 9001:2015 Certified Laboratory.

Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.

Small volumes of anti-DARS2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Description: ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp).

Subunit Structure: Homodimer.

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Type 1 subfamily.

Western Blot (WB)

WB: 1:500-1:2000

staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(1:200 Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(1:600 Green) were used as the secondary antibod

Western blot analysis of extracts from K-562 cells?, using DARS2 Antibody. The lane on the left was treated with blocking peptide.

Western blot analysis of extracts from Hybridoma cells, using DARS2 Antibody. The lane on the left was treated with blocking peptide.

Observed: 68 kDa
Predicted: 74 kDa

aspartyl-tRNA synthetase 2, mitochondrial

aspartate--tRNA ligase, mitochondrial

Aspartate--tRNA ligase, mitochondrial

AspRS??[Similar Products]

SYDM_HUMAN

The protein encoded by this gene belongs to the class-II aminoacyl-tRNA synthetase family. It is a mitochondrial enzyme that specifically aminoacylates aspartyl-tRNA. Mutations in this gene are associated with leukoencephalopathy with brainstem and spinal cord involvement and lactate elevation (LBSL). [provided by RefSeq, Nov 2009]

DARS2: Defects in DARS2 are a cause of leukoencephalopathy with brainstem and spinal cord involvement and lactate elevation (LBSL). LBSL is an autosomal recessive disease and is defined on the basis of a highly characteristic constellation of abnormalities observed by magnetic resonance imaging and spectroscopy. Affected individuals develop slowly progressive cerebellar ataxia, spasticity, and dorsal column dysfunction, sometimes with a mild cognitive deficit or decline. Belongs to the class-II aminoacyl-tRNA synthetase family.

Protein type: Ligase; Translation; EC 6.1.1.12; Mitochondrial

Chromosomal Location of Human Ortholog: 1q25.1

Cellular Component: nucleoplasm; mitochondrion; mitochondrial matrix; nucleus

Molecular Function: protein binding; protein homodimerization activity; aspartate-tRNA ligase activity; aspartate-tRNA(Asn) ligase activity; ATP binding; tRNA binding

Biological Process: tRNA aminoacylation for protein translation; gene expression; tRNA aminoacylation

Disease: Leukoencephalopathy With Brainstem And Spinal Cord Involvement And Lactate Elevation

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