Product Name
ASPA, Recombinant Protein
Full Product Name
ASPA, 1-313aa, Human, His tag, E Coli
Product Synonym Names
Aspartoacylase; ACY2; ASP
Product Gene Name
ASPA recombinant protein
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence
MGSSHHHHHH SSGLVPRGSH MGSMTSCHIA EEHIQKVAIF GGTHGNELTG VFLVKHWLEN GAEIQRTGLE VKPFITNPRA VKKCTRYIDC DLNRIFDLEN LGKKMSEDLP YEVRRAQEIN HLFGPKDSED SYDIIFDLHN TTSNMGCTLI LEDSRNNFLI QMFHYIKTSL APLPCYVYLI EHPSLKYATT RSIAKYPVGI EVGPQPQGVL RADILDQMRK MIKHALDFIH HFNEGKEFPP CAIEVYKIIE KVDYPRDENG EIAAIIHPNL QDQDWKPLHP GDPMFLTLDG KTIPLGGDCT VYPVFVNEAA YYEKKEAFAK TTKLTLNAKS IRCCLH
3D Structure
ModBase 3D Structure for P45381
Purity/Purification
> 90% by SDS-PAGE
Form/Format
Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 20% glycerol, 1mM DTT, 0.1M NaCl, 0.1mM PMSF
Concentration
0.5 mg/ml (determined by Bradford assay) (lot specific)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Other Notes
Small volumes of ASPA recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
ASPA recombinant protein
ASPA, also known as aspartoacylase, is expressed in liver, lung and kidney tissue, as well as in skeletal muscle and in cerebral white matter. Existing as a homodimer, Aspartoacylase functions to catalyze the deacetylation of N-acetylaspartic acid (NAA) (a protein whose hydrolysis is crucial to maintenance of intact white matter) to produce acetate and L-aspartate. Recombinant human ASPA protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography.
Product Categories/Family for ASPA recombinant protein
Neuroscience
Applications Tested/Suitable for ASPA recombinant protein
SDS-PAGE
SDS-Page of ASPA recombinant protein
NCBI/Uniprot data below describe general gene information for ASPA. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000040
[Other Products]
NCBI GenBank Nucleotide #
NM_000049.2
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UniProt Primary Accession #
P45381
[Other Products]
UniProt Related Accession #
P45381[Other Products]
Molecular Weight
38.1 kDa (336aa), confirmed by MALDI-TOF
NCBI Official Full Name
aspartoacylase
NCBI Official Synonym Full Names
aspartoacylase
NCBI Official Symbol
ASPA??[Similar Products]
NCBI Official Synonym Symbols
ASP; ACY2
??[Similar Products]
NCBI Protein Information
aspartoacylase
UniProt Protein Name
Aspartoacylase
UniProt Synonym Protein Names
Aminoacylase-2; ACY-2
Protein Family
Aspartocin
UniProt Gene Name
ASPA??[Similar Products]
UniProt Synonym Gene Names
ACY2; ASP; ACY-2??[Similar Products]
NCBI Summary for ASPA
This gene encodes an enzyme that catalyzes the conversion of N-acetyl_L-aspartic acid (NAA) to aspartate and acetate. NAA is abundant in the brain where hydrolysis by aspartoacylase is thought to help maintain white matter. This protein is an NAA scavenger in other tissues. Mutations in this gene cause Canavan disease. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for ASPA
Catalyzes the deacetylation of N-acetylaspartic acid (NAA) to produce acetate and L-aspartate. NAA occurs in high concentration in brain and its hydrolysis NAA plays a significant part in the maintenance of intact white matter. In other tissues it act as a scavenger of NAA from body fluids.
Product References and Citations for ASPA recombinant protein
Le Coq. et al. (2006) Biochemistry 45: 5878-5884.; Le Coq. et al. (2008) Biochemistry 47: 3484-3492.
Research Articles on ASPA
1. report of 2 Egyptian sibling patients suspected of Canavan disease (CD); study revealed homozygosity for substitution T530C (Ile177Thr) in exon 4 of the ASPA gene in both sibs; substitution T530C (Ile177Thr) results in a novel missense mutation causing a CD phenotype with severe clinical characteristics
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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