BHD; BHD skin lesion fibrofolliculoma protein; Birt Hogg Dube syndrome protein; Birt-Hogg-Dube syndrome protein; DKFZp547A118; FLCL; Flcn; FLCN_HUMAN; FLJ45004; FLJ99377; Folliculin; MGC17998; MGC23445

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

FLCN Antibody detects endogenous levels of FLCN.

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).

Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

1mg/ml (lot specific)

Store at -20 degree C. Stable for 12 months from date of receipt.

Manufactured in an ISO 9001:2015 Certified Laboratory.

Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.

Small volumes of anti-FLCN antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Description: May be a tumor suppressor. May be involved in energy and/or nutrient sensing through the AMPK and mTOR signaling pathways. May regulate phosphorylation of RPS6KB1.

Post Translational Modifications: Phosphorylated. Several different phosphorylated forms exist.

Subunit Structure: Interacts (via C-terminus) with FNIP1 and FNIP2 (via C-terminus). This mediates indirect interaction with the PRKAA1, PRKAB1 and PRKAG1 subunits of 5'-AMP-activated protein kinase (PubMed:17028174, PubMed:18403135, PubMed:18663353, PubMed:27353360). Interacts with HSP90AA1 in the presence of FNIP1. Interacts with HSP70, STUB1, CDC37, AHSA1, CCT2, STIP1, PTGES3 and PPP5C (PubMed:27353360).

Similarity: Belongs to the folliculin family.

Western Blot (WB)

WB: 1:500-1:2000

staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(1:200 Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(1:600 Green) were used as the secondary antibod

Western blot analysis of extracts from HeLa cells, using FLCN Antibody. The lane on the left was treated with blocking peptide.

Western blot analysis of extracts from various samples, using FLCN Antibody.
Lane 1: VERO cells, treated with blocking peptide;
Lane 2: VERO cells;
Lane 3: Mouse spleen;
Lane 4: MCF7.

Observed: 45 kDa; 70 kDa
Predicted: 65 kDa

folliculin

folliculin

Folliculin

BHD??[Similar Products]

This gene is located within the Smith-Magenis syndrome region on chromosome 17. Mutations in this gene are associated with Birt-Hogg-Dube syndrome, which is characterized by fibrofolliculomas, renal tumors, lung cysts, and pneumothorax. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

May be a tumor suppressor. May be involved in energy and/or nutrient sensing through the AMPK and mTOR signaling pathways. May regulate phosphorylation of RPS6KB1.

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