Product Name
CHAF1A, cDNA Clone
Full Product Name
CHAF1A cDNA Clone
Product Gene Name
CHAF1A cdna clone
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence
atgctggagg agctggagtg cggggcgccc ggcgccaggg gagccgccac agccatggat tgcaaagata gaccagcttt tccagttaag aagttaatac aagcccgtct gccgtttaag cgcctgaatc ttgtcccaaa ggggaaagcc gatgacatgt cagacgatca gggtacttct gtgcaaagta aaagccccga tttagaggcc tctttggaca ccttggaaaa caactgtcat gtgggttctg acatagactt tagaccgaaa cttgtcaacg ggaagggtcc cttagataac tttttaagaa atagaatcga aaccagtatt ggccagagca cagtcatcat tgatttgaca gaggactcga atgagcagcc agacagtctt gtggaccaca ataaactaaa ttctgaagcc tctccctcca gggaggcaat aaatggccag cgagaagaca ctggggatca gcaggggttg ttgaaggcca ttcagaacga caagttggca tttcctggag agaccctttc agacattcct tgcaaaacag aggaggaggg tgttggctgt ggaggtgcag ggaggagagg cgactcccag gaatgttcgc cacggagctg cccggagctg acgagtggcc cgagaatgtg ccccagaaag gagcaggaca gttggagtga agctgggggc atcctgttca aagggaaggt gcctatggtg gtcttgcagg acatcttggc tgtgagacca ccgcaaatca agtcccttcc agccacaccc caaggcaaga acatgacccc tgagagtgag gtgctggaat ctttccccga agaagactct gtactcagcc attcgtccct gagctctccc tcttccacca gctcgcccga ggggccgcct gctcccccaa agcagcacag cagtaccagt cccttcccca cctccacgcc cctccgcaga ataactaaga aattcgtcaa aggctctaca gagaagaaca agctcagact gcaaagagat caggagcgtc tgggcaagca gctcaagtta cgtgcagaaa gggaagaaaa ggagaagctg aaagaggagg ccaagcgggc caaggaggag gccaagaaga agaaggagga agagaaggag cttaaggaaa aggagaggcg ggagaagcgg gagaaggatg agaaggagaa ggcggagaag cagcggctca aggaggagcg gcgcaaggag agacaggaag ccctggaggc taaacttgag gaaaaaagga aaaaggaaga agagaaacgg ttaagagaag aagagaagcg cattaaagca gagaaggccg aaatcacgag gttcttccag aaaccaaaga ctccacaggc ccccaagacc ctggccggct cctgtgggaa gtttgccccc tttgaaatta aagagcacat ggtcctggcc cctcggcgtc ggaccgcttt ccatccagac ctctgcagtc agctggacca gctcctccag cagcagagcg gcgagttctc cttcttgaaa gacctcaaag gccggcagcc cctgaggtcc ggacccacgc acgtttccac ccggaatgca gatattttta acagtgatgt cgtcatcgtg gagcgtggga agggcgacgg tgttcccgag aggaggaagt ttggcaggat gaagctcctg cagttctgtg agaaccaccg gcctgcctac tggggtacct ggaataagaa gacggcactc atccgcgcgc gagacccctg ggcccaggac acgaagctcc tggactatga ggtggacagt gatgaggagt gggaagaaga ggagcctggg gagtccctgt cccacagtga gggggatgat gatgacgaca tgggagagga tgaagatgag gacgatggtt tctttgtgcc ccatgggtac ctgtctgagg acgaaggtgt gacagaggag tgtgccgacc ctgagaacca taaggtccgc cagaaactga aggccaagga gtgggacgag ttcctggcta aggggaagcg ctttcgcgtc ctgcaacctg tgaagatcgg ctgcgtgtgg gcggctgaca gagactgcgc aggcgatgac ctgaaggtac tgcagcagtt cgcagcctgc ttcctggaga ccctgccggc ccaggaggag cagacgccca aggcctccaa gcgggagagg agagacgagc agatcctggc ccagctgctg ccgctcctgc acggcaatgt gaacgggagc aaggtcatca tccgggagtt ccaggagcac tgccgccggg gactgctcag caaccacacc ggcagcccgc ggagcccctc caccacctac ctgcacaccc ccacccccag cgaggatgcc gccatcccct ctaagtcccg gctcaagcgg ctcatttccg agaactcagt gtatgagaag cggcctgact tcaggatgtg ctggtacgtg cacccgcagg tgctacagag cttccagcag gagcacctgc ccgtgccgtg ccagtggagc tatgtgacat cggtgccctc ggcccccaaa gaggacagtg gcagcgtccc ctccacgggg cccagccagg gcactcccat ctcgctgaag aggaagtcag cgggcagcat gtgcatcacc caattcatga agaagcgcag gcacgacggc cagattggtg ctgaagacat ggacggcttc caggcagaca cggaggagga ggaagaggag gagggcgact gtatgatcgt ggatgtcccg gatgctgcgg aggtccaagc cccgtgtgga gccgcttccg gagctggggg tggtgtgggg gtggacaccg gcaaggccac cctgaccgcg agcccactgg gtgcatcctg a
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of CHAF1A cdna clone vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
NCBI/Uniprot data below describe general gene information for CHAF1A. It may not necessarily be applicable to this product.
NCBI Accession #
BC067093
[Other Products]
UniProt Secondary Accession #
Q6NXG5; Q7Z7K3; Q9UJY8[Other Products]
UniProt Related Accession #
Q13111[Other Products]
Molecular Weight
88,285 Da
NCBI Official Full Name
Homo sapiens chromatin assembly factor 1, subunit A (p150), mRNA
NCBI Official Synonym Full Names
chromatin assembly factor 1 subunit A
NCBI Official Symbol
CHAF1A??[Similar Products]
NCBI Official Synonym Symbols
CAF1; P150; CAF-1; CAF1B; CAF1P150
??[Similar Products]
NCBI Protein Information
chromatin assembly factor 1 subunit A
UniProt Protein Name
Chromatin assembly factor 1 subunit A
UniProt Synonym Protein Names
Chromatin assembly factor I p150 subunit; CAF-I 150 kDa subunit; CAF-I p150; hp150
Protein Family
Chromatin assembly factor
UniProt Gene Name
CHAF1A??[Similar Products]
UniProt Synonym Gene Names
CAF; CAF1P150; CAF-1 subunit A; CAF-I 150 kDa subunit; CAF-I p150; hp150??[Similar Products]
UniProt Entry Name
CAF1A_HUMAN
NCBI Summary for CHAF1A
Chromatin assembly factor I (CAF1) is a nuclear complex consisting of p50, p60 (CHAF1B; MIM 601245), and p150 (CHAF1A) subunits that assembles histone octamers onto replicating DNA in vitro (Kaufman et al., 1995 [PubMed 7600578]).[supplied by OMIM, Mar 2008]
UniProt Comments for CHAF1A
CAF-1A: Core component of the CAF-1 complex, a complex thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. CHAF1A binds to histones H3 and H4. It may play a role in heterochromatin maintenance in proliferating cells by bringing newly synthesized cbx proteins to heterochromatic DNA replication foci. The CCR4-NOT complex functions as general transcription regulation complex. Also involved in vitamin D- coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR- mediated transrepression of the CYP27B1 gene. Homodimer. Part of the CAF-1 complex that contains RBBP4, CHAF1B and CHAF1A. CHAF1A binds directly to CHAF1B. Only minor amounts of RBBP4 are complexed with CHAF1A and CHAF1B in G1 phase. Part of the CCR4-NOT core complex that contains CHAF1A, CHAF1B, CNOT1, CNOT2, CNOT3, CNOT4, CNOT6 and CNOT8. CHAF1A binds directly to PCNA and to CBX1. Binds MBD1. Interacts directly with CBX5 via the PxVxL motif. During DNA replication, it forms a S phase- specific complex that facilitates DNA methylation and histone H3 'Lys-9' methylation during replication-coupled chromatin assembly and is at least composed of the CHAF1A, MBD1 and SETDB1. Component of the WINAC complex, at least composed of SMARCA2, SMARCA4, SMARCB1, SMARCC1, SMARCC2, SMARCD1, SMARCE1, ACTL6A, BAZ1B/WSTF, ARID1A, SUPT16H, CHAF1A and TOP2B. Interacts with CBX5. Belongs to the CHAF1A family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA replication
Chromosomal Location of Human Ortholog: 19p13.3
Cellular Component: intracellular membrane-bound organelle; nuclear chromatin; nucleolus; nucleus; protein complex
Molecular Function: chromatin binding; identical protein binding; protein binding; unfolded protein binding
Biological Process: chromatin assembly; DNA replication-dependent nucleosome assembly; protein complex assembly
Research Articles on CHAF1A
1. Taken together, these results show that CHAF1A contributes to the proliferation of glioblastoma cells and may be developed as a de novo drug target and prognosis biomarker of glioblastoma.
Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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