Full Product Name
VDR siRNA (Rat)
Product Synonym Names
NR1I1; Vitamin D3 receptor; VDR; 1.25-dihydroxyvitamin D3 receptor; Nuclear receptor subfamily 1 group I member 1
Product Gene Name
VDR sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P13053
Specificity
VDR siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat VDR gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of VDR sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
VDR sirna
siRNA to inhibit VDR expression using RNA interference
Applications Tested/Suitable for VDR sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for VDR. It may not necessarily be applicable to this product.
NCBI Accession #
NP_058754.1
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NCBI GenBank Nucleotide #
NM_017058.1
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UniProt Primary Accession #
P13053
[Other Products]
UniProt Related Accession #
P13053[Other Products]
Molecular Weight
47,814 Da
NCBI Official Full Name
vitamin D3 receptor
NCBI Official Synonym Full Names
vitamin D (1,25- dihydroxyvitamin D3) receptor
NCBI Official Symbol
Vdr??[Similar Products]
NCBI Official Synonym Symbols
Nr1i1
??[Similar Products]
NCBI Protein Information
vitamin D3 receptor
UniProt Protein Name
Vitamin D3 receptor
UniProt Synonym Protein Names
1,25-dihydroxyvitamin D3 receptor; Nuclear receptor subfamily 1 group I member 1
Protein Family
Vitamin D3 receptor
UniProt Gene Name
Vdr??[Similar Products]
UniProt Synonym Gene Names
Nr1i1; VDR??[Similar Products]
UniProt Entry Name
VDR_RAT
NCBI Summary for VDR
binds vitamin D3; acts as a transcriptional regulator of the osteocalcin gene [RGD, Feb 2006]
UniProt Comments for VDR
VDR: Nuclear hormone receptor. Transcription factor that mediates the action of vitamin D3 by controlling the expression of hormone sensitive genes. Regulates transcription of hormone sensitive genes via its association with the WINAC complex, a chromatin-remodeling complex. Recruited to promoters via its interaction with the WINAC complex subunit BAZ1B/WSTF, which mediates the interaction with acetylated histones, an essential step for VDR-promoter association. Plays a central role in calcium homeostasis. Defects in VDR are the cause of rickets vitamin D- dependent type 2A (VDDR2A). A disorder of vitamin D metabolism resulting in severe rickets, hypocalcemia and secondary hyperparathyroidism. Most patients have total alopecia in addition to rickets. Belongs to the nuclear hormone receptor family. NR1 subfamily.
Protein type: DNA-binding; Transcription factor; Nuclear receptor
Cellular Component: heterochromatin; nuclear matrix; intracellular membrane-bound organelle; cell; T-tubule; caveola; nuclear heterochromatin; cytosol; nucleoplasm; euchromatin; dense fibrillar component; perinuclear region of cytoplasm; nucleus; receptor complex
Molecular Function: retinoid X receptor binding; vitamin D binding; protein binding; ligand-dependent nuclear receptor activity; DNA binding; zinc ion binding; sequence-specific DNA binding; steroid hormone receptor activity; vitamin D3 receptor activity; transcription factor activity
Biological Process: lactation; transcription from RNA polymerase II promoter; heart development; multicellular organismal development; cell morphogenesis; positive regulation of apoptosis involved in mammary gland involution; negative regulation of transcription from RNA polymerase II promoter; decidualization; response to estradiol stimulus; intestinal absorption; negative regulation of cell proliferation; regulation of transcription, DNA-dependent; calcium ion transport; skeletal development; positive regulation of keratinocyte differentiation; aging; intracellular receptor-mediated signaling pathway; transcription, DNA-dependent; regulation of calcium ion transport; cellular calcium ion homeostasis; regulation of transcription from RNA polymerase II promoter; organ morphogenesis; positive regulation of transcription from RNA polymerase II promoter; steroid hormone mediated signaling; response to calcium ion; negative regulation of transcription, DNA-dependent
Research Articles on VDR
1. Thus, VDR in the apical brush border of the proximal convoluted tubule cells serves to "sense" the level of circulating 1,25(OH)2D3 and modulates the activity of the 1alpha-hydroxylase and the 24-hydroxylase accordingly.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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