Zebrafish Testicular Feeder Cells (ZtA6-5)
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產品名稱: Zebrafish Testicular Feeder Cells (ZtA6-5)
產品型號: T2604
產品展商: 其他品牌
產品文檔: 無相關文檔
簡單介紹
Zebrafish Testicular Feeder Cells (ZtA6-5)
Zebrafish Testicular Feeder Cells (ZtA6-5)
的詳細介紹
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Print Version |
| Safety |
BioSafety Level II |
| Organism |
Albino-type (alb-1/alb-1) zebrafish (Danio rerio) |
| Source Organ |
Testis |
| Growth Properties |
Adherent |
| Morphology |
Epithelial and fibroblast-like |
| Applications |
For Research Use Only. |
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| Description |
ZtA6 cells are derived from spontaneous tumor-like hypertropheied testis isolated from albino-type (alb-1/alb-1) zebrafish. Clones were analyzed for the expression of the Sertoli cell marker (Sox9a), the germ cell marker (Vas), and the Wilm’s tumor suppressor marker (WT1). 12 clones were derived and have distinctive properties and are available at abm.
ZtA6-5 is clone 5 derived from the ZtA6 zebrafish testicular cells which express Sox9a and Vas markers. Among the 12 clones derived from the ZtA6 cells, ZtA6-5 showed high levels of phagocytic activity. The ZtA6-5 cells may be used for further genetic manipulation and is a suitable tool for research in in vitro fertilization and vertebrate spermatogenesis as it is able to support the differentiation of germ cells into functional sperm.
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| Quality Control |
1) Phagocytic activity was analyzed via the ability to internalize polystyrene beads;
2) RT-PCR was used to assess the presence or absence of WT1, Vas, and Sox9a markers;
3) Functionality test was performed to determine the ability to support male germ cells when co-cultured as feeder cells. |
| Propagation |
Grow cells in T25 gelatin-coated flasks (TM063) with the following conditions. The base medium for this cell line is L-15 medium (ThermoFisher Scientific). To make the completed growth medium, add the following components to the base medium at the following final concentrations: 5 IU/ml human chorionic gonadotropin (Sigma), 2 IU/ml pregnant mare’s serum gonadotropin (Sigma), 0.2 mg/ml L-arginine (Gibco BRL), 0.02 mg/ml L-aspartic acid (Gibco BRL), 0.015 mg/ml L-histidine (Gibco BRL), 0.0725 mg/ml L-lysine HCl (Gibco BRL), 0.02 mg/ml L-proline (Gibco BRL), 0.5% bovine serum albumin (5% w/v stock solution, BSA fraction V, Sigma), 1% Hepes (Sigma), 3% fetal bovine serum (TM999), and 1% Penicillin/Streptomycin (G255). Atmosphere: air: 100%, Temperature: 28.0°C.
To prepare as feeder cells: Treat with 10 μg/ml mitomycin C in L-15 for 5 hours before plating for use as feeder cells for further experimentation.
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| Storage |
-180°C |
| Shipping |
On Dry Ice |
| Notes |
For Research Use Only, not for therapeutic or diagnostic purposes. |
