mCLING labeling kit - 710-MCK
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產(chǎn)品名稱: mCLING labeling kit - 710-MCK
產(chǎn)品型號(hào): 710-MCK
產(chǎn)品展商: 其他品牌
產(chǎn)品文檔: 無(wú)相關(guān)文檔
簡(jiǎn)單介紹
mCLING labeling kit - 710-MCK
mCLING labeling kit - 710-MCK
的詳細(xì)介紹
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Cat. No. 710-MCK
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Content:
710-MCK A: unlabeled mCling, 50 nmol (lyophilized)
710-MCK B: mCLING purification column
710-MCK C: Washing buffer, 10 ml
710-MCK D: Stop solution, 0.5 ml
710-MCK E: Elution buffer, 1 ml
710-MCK F: Neutralization buffer, 0.5 ml
710-mck.pdf
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Storage
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All components of the kit are stable at 2-8°C.
Shelf life before use: 6 months.
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Applications
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WB: (see remarks)
ICC: yes
IHC: yes
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Remarks
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WB: Due to the positive charge of mCLING, negatively charged coatings of cover-slips should be avoided. We recommend a positively charged coating like poly-L-lysine (PLL). mCLING is a fixable dye but paraformaldehyde alone is not able to fix this molecule sufficiently. Therefore, a mixture of 4 %paraformaldehyde (PFA) and 0.2 % glutaraldehyde is strongly advised. For detailed protocols see Revelo NH & Rizzoli SO, 2016.
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Datasheet
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710-mck.pdf
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