Glycine receptor - 146 308
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產(chǎn)品名稱: Glycine receptor - 146 308
產(chǎn)品型號(hào): 146 308
產(chǎn)品展商: 其他品牌
產(chǎn)品文檔: 無(wú)相關(guān)文檔
簡(jiǎn)單介紹
Glycine receptor - 146 308
Glycine receptor - 146 308
的詳細(xì)介紹
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Cat. No. 146 308
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50 μg purified IgG, lyophilized. For reconstitution add 50 μl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
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Applications
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WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: not tested yet IHC: 1 : 500 (see remarks) gallery IHC-P/FFPE: not tested yet
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Clone
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GpmAb4a
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Subtype
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IgG2 (κ light chain)
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Immunogen
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Recombinant protein corresponding to AA 1 to 457 from rat Glycine receptor α1 (UniProt Id: P07727)
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Epitop
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Epitop: AA 96 to 105 from rat Glycine receptor α1 (UniProt Id: P07727)
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Reactivity
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Reacts with: human (P23415, P23416, P48167 ), rat (P07727, P22771, P20781 ), mouse (Q64018, Q7TNC8, P48168 ), pig, zebrafish. Other species not tested yet.
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Specificity
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Specific for all glycine receptor subunits.
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Remarks
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This antibody is a chimeric antibody based on the well known monoclonal mouse antibody mAb4a. The constant regions of the heavy and light chains have been replaced with Guinea pig specific sequences. The antibody can therefore be used with standard anti-Guinea pig secondary reagents. It also carries a Strep-tag at the C-terminus of the heavy chain.The antibody has been expressed in mammalian cells. IHC: Tissue sections require additional methanol/acetic acid treatment prior to antibody incubation. For details see Dumoulin A, Triller A & Dieudonné S (2001).
recommended protocol
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Datasheet
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146_308.pdf
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