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產(chǎn)品名稱: Gephyrin - 147 018
產(chǎn)品型號: 147 018
產(chǎn)品展商: 其他品牌
產(chǎn)品文檔: 無相關(guān)文檔
簡單介紹
Gephyrin - 147 018
Gephyrin - 147 018
的詳細(xì)介紹
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Cat. No. 147 018
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200 μl hybridoma supernatant, lyophilized. For reconstitution add 200 μl H2O, then aliquot and store at -20°C until use.
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Applications
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WB: not recommended (see remarks) IP: not recommended (see remarks) ICC: 1 : 1000 gallery IHC: 1 : 500 up to 1 : 1000 (see remarks) gallery IHC-P/FFPE: not tested yet
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Clone
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RbmAb7a
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Subtype
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IgG1 (κ light chain)
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Immunogen
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Recombinant protein corresponding to AA 1 to 768 from rat Gephyrin (UniProt Id: Q03555)
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Epitop
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N-terminus of gephyrin, surrounding phospho-serines 268 and 270.
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Reactivity
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Reacts with: human (Q9NQX3), rat (Q03555), mouse (Q8BUV3), pig, goldfish, zebrafish. Other species not tested yet.
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Specificity
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Specific for the brain specific 93 kDa splice variant. (K.O. verified)
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Remarks
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WB: Clone 3B11 (cat. no. 147 111) highly recommended. IP: Clone 3B11 (cat. no. 147 111) highly recommended. IHC: For best results use the protocol of Schneider Gasser et al., 2006.
Alternatively AGR (10 mM citrate, pH 6.0, over night at 60°C) can be applied.
This antibody is a chimeric antibody based on the well known monoclonal mouse antibody mAb7a. The constant regions of the heavy and light chains have been replaced with rabbit specific sequences. The antibody can therefore be used with standard anti-rabbit secondary reagents.
The antibody has been expressed in mammalian cells.
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Datasheet
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147_018.pdf
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